{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:14:20Z","timestamp":1790194460549,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2016,7,13]],"date-time":"2016-07-13T00:00:00Z","timestamp":1468368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["254256"],"award-info":[{"award-number":["254256"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:p>R\u2010spondin3 (Rspo3) is a secreted protein, which acts as an agonist of canonical Wnt\/\u03b2\u2010catenin signaling that plays an important role in embryonic development and homeostasis. In this study, we focused on <jats:italic>C<\/jats:italic>\u2010mannosylation, a unique type of glycosylation, of human Rspo3. Rspo3 has two putative <jats:italic>C<\/jats:italic>\u2010mannosylation sites at Trp<jats:sup>153<\/jats:sup> and Trp<jats:sup>156<\/jats:sup>; however, it had been unclear whether these sites are <jats:italic>C<\/jats:italic>\u2010mannosylated or not. We demonstrated that Rspo3 was <jats:italic>C<\/jats:italic>\u2010mannosylated at both Trp<jats:sup>153<\/jats:sup> and Trp<jats:sup>156<\/jats:sup> by mass spectrometry. Using <jats:italic>C<\/jats:italic>\u2010mannosylation\u2010defective Rspo3 mutant\u2010overexpressing cell lines, we found that <jats:italic>C<\/jats:italic>\u2010mannosylation of Rspo3 promotes its secretion and activates Wnt\/\u03b2\u2010catenin signaling.<\/jats:p>","DOI":"10.1002\/1873-3468.12274","type":"journal-article","created":{"date-parts":[[2016,6,28]],"date-time":"2016-06-28T11:46:39Z","timestamp":1467114399000},"page":"2639-2649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["<i>C<\/i>\u2010mannosylation of R\u2010spondin3 regulates its secretion and activity of Wnt\/\u03b2\u2010catenin signaling in cells"],"prefix":"10.1002","volume":"590","author":[{"given":"Miho","family":"Fujiwara","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Technology Keio University Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shintaro","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Technology Keio University Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Niwa","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Technology Keio University Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiro","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Biomolecular Characterization Unit RIKEN Center for Sustainable Resource Science Wako Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyu","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Technology Keio University Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Sasazawa","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Technology Keio University Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoshi","family":"Dohmae","sequence":"additional","affiliation":[{"name":"Biomolecular Characterization Unit RIKEN Center for Sustainable Resource Science Wako Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siro","family":"Simizu","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Technology Keio University Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.14.1.59"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5482.1194"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/73448"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/79859"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805778105"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/embj.201385358"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.15.1837"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbaexp.2003.10.009"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-02-0187"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508324200"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00272-12"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2011.175"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2013.167"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwm050"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008073200"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/9.5.435"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.03.003"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng2034"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15-06-0373"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.08.001"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2014.2438"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2014.06.001"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2014.10.004"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.606293"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-1053"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.011015"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(90)90238-5"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2013.1771"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00240-9"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01611"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.027284"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702305104"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.10.116"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310490110"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040976"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-0561"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F1873-3468.12274","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.12274","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T07:26:05Z","timestamp":1694935565000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.12274"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,13]]},"references-count":36,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["10.1002\/1873-3468.12274"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.12274","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,13]]},"assertion":[{"value":"2016-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}