{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:26:47Z","timestamp":1791293207344,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2016,8,9]],"date-time":"2016-08-09T00:00:00Z","timestamp":1470700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p>Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2 is a chromatin\u2010associated protein that is mutated in Rett syndrome. Its methyl\u2010CpG\u2010binding domain interacts with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> containing methylated cytosine, but other modes of recruitment to the genome have also been proposed. Here, we use <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> assays to investigate the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> binding specificity of two <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\u2010hook motifs in Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2. One exhibits robust sequence\u2010specific <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> binding, whereas the other is a much weaker <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\u2010hook. Our data indicate that these motifs are secondary contributors to <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> binding by Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2, and this view is supported by the absence of disease\u2010causing missense mutations at these sites.<\/jats:p>","DOI":"10.1002\/1873-3468.12328","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T08:14:18Z","timestamp":1469607258000},"page":"2927-2933","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Sequence\u2010specific <scp>DNA<\/scp> binding by <scp>AT<\/scp>\u2010hook motifs in Me<scp>CP<\/scp>2"],"prefix":"10.1002","volume":"590","author":[{"given":"Matthew J.","family":"Lyst","sequence":"first","affiliation":[{"name":"The Wellcome Trust Centre for Cell Biology University of Edinburgh Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Connelly","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Centre for Cell Biology University of Edinburgh Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cara","family":"Merusi","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Centre for Cell Biology University of Edinburgh Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Bird","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Centre for Cell Biology University of Edinburgh Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/13810"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90610-O"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.21.4886"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3607"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14319"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.01.030"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.011"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.12.028"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.9.5656"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.01.038"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.19.4413"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38926-4"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0897-657"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.07.021"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M803021200"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3434"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2015.118"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037120"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3897"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F1873-3468.12328","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.12328","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T22:28:12Z","timestamp":1694903292000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.12328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,9]]},"references-count":19,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1002\/1873-3468.12328"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.12328","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,9]]},"assertion":[{"value":"2016-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-08-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}