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In addition, we investigated the hydroxylation of 11\u2010deoxycorticosterone by <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>260A1 by reconstituting the enzyme with the surrogate redox partners adrenodoxin and adrenodoxin reductase. The major product of this steroid conversion was identified as 1\u03b1\u2010hydroxy\u201011\u2010deoxycorticosterone, a novel \u03944 C\u201021 steroidal derivative. Furthermore, we docked the substrate into the crystal structure and replaced Ser326, the residue responsible for substrate orientation, with asparagine and observed that the mutant S326N displayed higher activity and selectivity for the formation of 1\u03b1\u2010hydroxy\u201011\u2010deoxycorticosterone compared to the wild\u2010type <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>260A1. Thus, our findings highlight the usefulness of the obtained crystal structure of <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>260A1 in identifying biotechnologically more efficient reactions.<\/jats:p>","DOI":"10.1002\/1873-3468.12479","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T16:00:55Z","timestamp":1478534455000},"page":"4638-4648","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Structural characterization of <scp>CYP<\/scp>260A1 from <i>Sorangium cellulosum<\/i> to investigate the 1\u03b1\u2010hydroxylation of a mineralocorticoid"],"prefix":"10.1002","volume":"590","author":[{"given":"Yogan","family":"Khatri","sequence":"first","affiliation":[{"name":"Institute of Biochemistry Saarland University Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Carius","sequence":"additional","affiliation":[{"name":"Department of Structural Biology Institute of Biophysics and Center of Human and Molecular Biology (ZHMB) Saarland University Homburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Ringle","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry Saarland University Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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