{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:40:33Z","timestamp":1761896433036},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T00:00:00Z","timestamp":1482105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102013M3A9A7046297"],"award-info":[{"award-number":["NRF\u20102013M3A9A7046297"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Previously, a surgical regression model identified micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010101b (<jats:italic>miR\u2010101b<\/jats:italic>) as a potential inhibitor of cardiac hypertrophy. Here, we investigated the antihypertrophic mechanism of <jats:italic>miR\u2010101b<\/jats:italic> using neonatal rat ventricular myocytes. <jats:italic>miR\u2010101b<\/jats:italic> markedly suppressed agonist\u2010induced cardiac hypertrophy as shown by cell size and fetal gene expression. By systems biology approaches, we identified protein kinase C epsilon (<jats:styled-content style=\"fixed-case\">PKC<\/jats:styled-content>\u03b5) as the major target of <jats:italic>miR\u2010101b<\/jats:italic>. Our results from <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, western blot, and luciferase reporter assays confirm that <jats:styled-content style=\"fixed-case\">PKC<\/jats:styled-content>\u03b5 is a direct target of <jats:italic>miR\u2010101b<\/jats:italic>. In addition, we found that effectors downstream of <jats:styled-content style=\"fixed-case\">PKC<\/jats:styled-content>\u03b5 (p\u2010<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>, p\u2010<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2, p\u2010<jats:styled-content style=\"fixed-case\">NFAT<\/jats:styled-content>, and p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2) are also affected by <jats:italic>miR\u2010101b<\/jats:italic>. Our study reveals a novel inhibitory mechanism for <jats:italic>miR\u2010101b<\/jats:italic> as a negative regulator of cardiac hypertrophy.<\/jats:p>","DOI":"10.1002\/1873-3468.12508","type":"journal-article","created":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T16:02:50Z","timestamp":1481126570000},"page":"16-27","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Micro<scp>RNA<\/scp>\u2010101b attenuates cardiomyocyte hypertrophy by inhibiting protein kinase C epsilon signaling"],"prefix":"10.1002","volume":"591","author":[{"given":"Jong Sub","family":"Lee","sequence":"first","affiliation":[{"name":"College of Life Sciences Gwangju Institute of Science and Technology Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Kwon","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Gwangju Institute of Science and Technology Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jei Hyoung","family":"Park","sequence":"additional","affiliation":[{"name":"College of Life Sciences Gwangju Institute of Science and Technology Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Ock","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Life Sciences Gwangju Institute of Science and Technology Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo Jin","family":"Park","sequence":"additional","affiliation":[{"name":"College of Life Sciences Gwangju Institute of Science and Technology Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunghee","family":"Cho","sequence":"additional","affiliation":[{"name":"College of Life Sciences Gwangju Institute of Science and Technology Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Do Han","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Life Sciences Gwangju Institute of Science and Technology Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,12,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1983"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199910213411706"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)41010-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.279.3.L429"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.100.18.1909"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.33.20317"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.11.1104"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.3.264"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a003296"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0351040"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2013-305402"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000257913.42552.23"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.300226"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.10.040"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136236"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20130833"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)01018-3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035552"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000072977.86706.23"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.10R28FM"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707659"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.109637"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2010.10.020"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199005313222203"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.65.092101.142243"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199001113220206"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17050749"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.202176"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1582"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0122509"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.094524"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.12.1218"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00475.2003"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200108062"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00408.2001"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.06.017"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules200610435"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24682"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F1873-3468.12508","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.12508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T01:31:05Z","timestamp":1694827865000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.12508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,19]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1002\/1873-3468.12508"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.12508","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,19]]},"assertion":[{"value":"2016-09-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}