{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:37:27Z","timestamp":1775237847734,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T00:00:00Z","timestamp":1505952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p>Scorpion \u03b1\u2010toxins are polypeptides that inhibit voltage\u2010gated sodium channel inactivation. They are divided into mammal, insect and \u03b1\u2010like toxins based on their relative activity toward different phyla. Several factors are currently known to influence the selectivity, which are not just particular amino acid residues but also general physical, chemical, and topological properties of toxin structural modules. The objective of this study was to change the selectivity profile of a chosen broadly active \u03b1\u2010like toxin, BeM9 from <jats:italic>Mesobuthus eupeus<\/jats:italic>, toward mammal\u2010selective. Based on the available information on what determines scorpion \u03b1\u2010toxin selectivity, we designed and produced msBeM9, a BeM9 derivative, which was verified to be exclusively active toward mammalian sodium channels and, most importantly, toward the Na<jats:sub>v<\/jats:sub>1.2 isoform expressed in the brain.<\/jats:p>","DOI":"10.1002\/1873-3468.12839","type":"journal-article","created":{"date-parts":[[2017,9,10]],"date-time":"2017-09-10T11:42:36Z","timestamp":1505043756000},"page":"3414-3420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Design of sodium channel ligands with defined selectivity \u2013 a case study in scorpion alpha\u2010toxins"],"prefix":"10.1002","volume":"591","author":[{"given":"Nikita A.","family":"Kuldyushev","sequence":"first","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Moscow Russia"},{"name":"Moscow Institute of Physics and Technology (State University) Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonina A.","family":"Berkut","sequence":"additional","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Moscow Russia"},{"name":"Moscow Institute of Physics and Technology (State University) Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Peigneur","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology University of Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Tytgat","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology University of Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene V.","family":"Grishin","sequence":"additional","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander A.","family":"Vassilevski","sequence":"additional","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.09.006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep14973"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.14.8034"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.09.023"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201310998"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201411268"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.431650"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211931200"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112320108"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.11.016"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9528309"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.23.14810"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.02918.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0270438"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.06.067"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402048200"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2485fje"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.08.068"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05737.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.021303"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msp310"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb400012k"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336950.2015.1120392"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw065"},{"key":"e_1_2_8_27_1","first-page":"979","article-title":"Amino acid sequence of 2 neurotoxins from the scorpion Buthus eupeus venom","volume":"10","author":"Volkova TM","year":"1984","journal-title":"Bioorg Khim"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2010.07.023"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160436"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00217a007"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2012.08545.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b00067"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-4622(88)80016-4"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1972.tb01924.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-003-2534-2"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.012054"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F1873-3468.12839","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.12839","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T03:42:57Z","timestamp":1696563777000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.12839"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,21]]},"references-count":35,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1002\/1873-3468.12839"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.12839","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,21]]},"assertion":[{"value":"2017-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}