{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:05:55Z","timestamp":1788437155932,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2017,10,19]],"date-time":"2017-10-19T00:00:00Z","timestamp":1508371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R15AI099897\u201001A1"],"award-info":[{"award-number":["1R15AI099897\u201001A1"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004834","name":"Universitat Jaume I","doi-asserted-by":"publisher","award":["P1.1B2015\u201028"],"award-info":[{"award-number":["P1.1B2015\u201028"]}],"id":[{"id":"10.13039\/501100004834","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:p>Tight regulation of <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> is critical for the structure and function of cells and organelles. The <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> environment changes dramatically along the endocytic pathway, an internalization transport process that is \u2018hijacked\u2019 by many intracellularly active bacterial exotoxins, including the anthrax toxin. Here, we investigate the role of <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> on single\u2010channel properties of the anthrax toxin protective antigen (<jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content><jats:sub>63<\/jats:sub>). Using conductance and current noise analysis, blocker binding, ion selectivity, and poly(ethylene glycol) partitioning measurements, we show that the channel exists in two different open states (\u2018maximum\u2019 and \u2018main\u2019) at <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> \u2265\u00a05.5, while only a maximum conductance state is detected at <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\u00a0&lt;\u00a05.5. We describe two substantially distinct patterns of <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content><jats:sub>63<\/jats:sub> conductance dependence on <jats:styled-content style=\"fixed-case\">KCl<\/jats:styled-content> concentration uncovered at <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 6.5 and 4.5.<\/jats:p>","DOI":"10.1002\/1873-3468.12866","type":"journal-article","created":{"date-parts":[[2017,9,30]],"date-time":"2017-09-30T14:26:34Z","timestamp":1506781594000},"page":"3481-3492","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of endosomal acidification on small ion transport through the anthrax toxin <scp>PA<\/scp><sub>63<\/sub> channel"],"prefix":"10.1002","volume":"591","author":[{"given":"Nnanya","family":"Kalu","sequence":"first","affiliation":[{"name":"Department of Biology The Catholic University of America Washington DC 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