{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T21:42:04Z","timestamp":1778622124108,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,3,2]],"date-time":"2018-03-02T00:00:00Z","timestamp":1519948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["675179"],"award-info":[{"award-number":["675179"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>The ubiquitin\u2010specific protease 8 (USP8)\/14\u20103\u20103 protein\u2013protein interaction has recently been shown to exert a significant role in the pathogenesis of Cushing's disease (CD). USP8 is a deubiquitinase that prevents epidermal growth factor receptor (EGFR) degradation. Impairment of 14\u20103\u20103 binding leads to a higher deubiquitination of EGFR and results in a higher EGFR signaling and an increased production of adrenocorticotropic hormone. Here we report the high\u2010resolution crystal structure of the 14\u20103\u20103 binding motif of USP8 surrounding Ser718 in complex with 14\u20103\u20103\u03b6 and characterize the interaction with fluorescence polarization and isothermal titration calorimetry. Furthermore, we analyze the effect of USP8 mutations identified in CD on binding to 14\u20103\u20103.<\/jats:p>","DOI":"10.1002\/1873-3468.13017","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T13:32:45Z","timestamp":1519392765000},"page":"1211-1220","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Biophysical and structural insight into the USP8\/14\u20103\u20103 interaction"],"prefix":"10.1002","volume":"592","author":[{"given":"Federica","family":"Centorrino","sequence":"first","affiliation":[{"name":"Laboratory of Chemical Biology Department of Biomedical Engineering Institute for Complex Molecular Systems Eindhoven University of Technology The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Ballone","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Biology Department of Biomedical Engineering Institute for Complex Molecular Systems Eindhoven University of Technology The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madita","family":"Wolter","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Biology Department of Biomedical Engineering Institute for Complex Molecular Systems Eindhoven University of Technology The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Ottmann","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Biology Department of Biomedical Engineering Institute for Complex Molecular Systems Eindhoven University of Technology The Netherlands"},{"name":"Department of Chemistry University of Duisburg\u2010Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61375-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1227\/NEU.0000000000001011"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2015.08.003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.2010.01406.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-06-0560"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.01.003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3166"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI60417"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-015-0353-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2017-00161"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290291"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2015.09.012"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bat085"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910048675"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913000061"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889897006766"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1107\/S090744499801405X"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci200227u"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80487-0"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2011.09.001"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605779103"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309110025479"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-12-127795"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01636-09"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08445.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90396-4"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.78.082307.091526"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.016"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2012.11.028"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2017.11.010"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400698c"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2017.1346608"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b00574"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2329"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867324666170426095015"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb4003464"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2013.03.015"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20102037"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201500484"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201507976"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201310082"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1570"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b07939"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F1873-3468.13017","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.13017","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1873-3468.13017","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.13017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T11:51:46Z","timestamp":1694346706000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.13017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,2]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1002\/1873-3468.13017"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.13017","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,2]]},"assertion":[{"value":"2017-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}