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However, multiple genetic manipulations are restricted by the paucity of selectable markers. The <jats:italic>bsd<\/jats:italic>\u2010blasticidin selection system is widely used for eukaryotic cells; however, it could not previously be used for <jats:italic>P. berghe<\/jats:italic>i due to toxicity to the rodent host. Here, we report the application of this selection system in <jats:italic>P. berghei<\/jats:italic> using an <jats:italic>in vitro<\/jats:italic> selection method. The desired <jats:italic>bsd\u2010<\/jats:italic>integrated mutants are enriched by more than 90% within 2 weeks when using this system. Furthermore, the <jats:italic>bsd<\/jats:italic> marker can be used sequentially with established pyrimethamine\u2010 and puromycin\u2010resistant markers. 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