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In this study, miR\u2010342\u20103p was found to be deregulated with ossification of ligament or osteoporosis. We demonstrate that silencing miR\u2010342\u20103p impairs osteoblast activity and matrix mineralization, while over expression of miR\u2010342\u20103p promotes osteoblast differentiation significantly. Moreover, miR\u2010342\u20103p directly targets activating transcription factor 3 (<jats:styled-content style=\"fixed-case\">ATF<\/jats:styled-content>3), which inhibits transcription of pro\u2010osteogenic differentiation\u2010associated genes. In addition, there exists a higher frequency of methylation at the CpG island of the Enah\/Vasp\u2010Like (<jats:styled-content style=\"fixed-case\">EVL<\/jats:styled-content>) locus in undifferentiated pre\u2010osteoblasts; however, demethylation of the <jats:styled-content style=\"fixed-case\">EVL<\/jats:styled-content> CpG island induces over expression of miR\u2010342\u20103p during osteogenic differentiation. This study suggests that miR\u2010342\u20103p may serves as a potential marker for diagnosis and treatment of ossification of ligament and osteoporosis.<\/jats:p>","DOI":"10.1002\/1873-3468.13282","type":"journal-article","created":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T08:58:53Z","timestamp":1541062733000},"page":"4051-4065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["miR\u2010342\u20103p promotes osteogenic differentiation via targeting <scp>ATF<\/scp>3"],"prefix":"10.1002","volume":"592","author":[{"given":"Yawei","family":"Han","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences Tianjin Medical University China"},{"name":"Department of Orthopaedics Surgery Tianjin Medical University General Hospital Tianjin Medical University 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