{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T22:14:46Z","timestamp":1771798486582,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Eastern Michigan University Provost Research Support Award\/Chemistry Seller's Fund"},{"name":"EMU Faculty Research Fellowship"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>Dysfunction of the dopaminergic pathway is linked to numerous diseases of the nervous system. The D1\u2013D2 receptor heteromer is known to play a role in certain neuropsychiatric disorders, such as depression. Here, we synthesized an eight amino acid residue peptide, <jats:styled-content style=\"fixed-case\">EAARRAQE<\/jats:styled-content>, derived from the third intracellular loop of the D2 receptor and show that the peptide binds to the D1 receptor with comparable efficiency as that of the full\u2010length D2 receptor protein. Moreover, immunoprecipitation studies show the existence of a heteromeric complex formed both <jats:italic>in\u00a0vitro<\/jats:italic> and in total protein derived from temporal and frontal lobe tissue from normal and depressed subjects. The efficiency of the peptide to block the D1\u2013D2 heteromeric complex was comparable in all the samples tested.<\/jats:p>","DOI":"10.1002\/1873-3468.13351","type":"journal-article","created":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T12:34:53Z","timestamp":1551443693000},"page":"732-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterizing the binding of dopamine D1\u2013D2 receptors <i>in\u00a0vitro<\/i> and in temporal and frontal lobe tissue total protein"],"prefix":"10.1002","volume":"593","author":[{"given":"Deborah L.","family":"Heyl","sequence":"first","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Champion","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Muterspaugh","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Connolly","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Baraka","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pouya","family":"Khazaei","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Briana","family":"Moe","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeinab","family":"Al\u2010Sheemary","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nael","family":"Jaber","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hedeel","family":"Guy\u2010Evans","sequence":"additional","affiliation":[{"name":"Chemistry Department Eastern Michigan University Ypsilanti MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1134\/S199075081702010X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2011.00064"},{"key":"e_1_2_8_4_1","first-page":"182671","article-title":"Central dopaminergic system and its implications in stress\u2010mediated neurological disorders and gastric ulcers: short review","volume":"2010","author":"Rasheed N","year":"2012","journal-title":"Adv Pharmacol Sci"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyx056"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00260"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2011.00031"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.05.022"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-1931(00)00025-2"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan5468"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.002642"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-420118-7.00008-1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.09.023"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604049104"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401923200"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.159954"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41432"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2013.148"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-6606-4-26"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.11.027"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-254037"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.113.085175"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2263"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.115634"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euprot.2014.05.003"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2015.00211"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(81)90322-8"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F1873-3468.13351","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.13351","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1873-3468.13351","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.13351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T09:20:11Z","timestamp":1694337611000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.13351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,12]]},"references-count":26,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/1873-3468.13351"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.13351","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,12]]},"assertion":[{"value":"2019-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}