{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:59:56Z","timestamp":1790521196489,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T00:00:00Z","timestamp":1563926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>The hybrid cluster protein (Hcp) contains a unique 4Fe cluster that is a hybrid of \u03bc\u2010S and \u03bc\u2010O bridges. <jats:italic>Escherichia\u00a0coli<\/jats:italic> Hcp has recently been found to carry NO reductase activity as well as <jats:italic>S<\/jats:italic>\u2010nitrosylation activity in NO\u2010based signaling. In other species, the physiological activity has not been established. No reaction mechanism of any Hcp has been proposed. Here, we show that <jats:italic>Desulfovibrio\u00a0vulgaris<\/jats:italic> (Hildenborough) Hcp has nitric oxide reductase activity with benzyl viologen as electron donor. With EPR spectroscopy, we identify three unexpected putative reaction intermediates: both in reduced and oxidized Hcp, dinitrosyl iron complexes are formed. Also, the hybrid cluster in reduced Hcp, but not in oxidized Hcp, binds the product N<jats:sub>2<\/jats:sub>O. Possible implications for a reaction mechanism are discussed.<\/jats:p>","DOI":"10.1002\/1873-3468.13539","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T14:25:06Z","timestamp":1563459906000},"page":"3075-3083","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["EPR spectroscopy of putative enzyme intermediates in the NO reductase and the auto\u2010nitrosylation reaction of <i>Desulfovibrio vulgaris<\/i> hybrid cluster protein"],"prefix":"10.1002","volume":"593","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1609-6671","authenticated-orcid":false,"given":"Wilfred R.","family":"Hagen","sequence":"first","affiliation":[{"name":"Department of Biotechnology Delft University of Technology Delft the 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