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The host ceramide transport protein CERT delivers ceramide from the endoplasmic reticulum to the Golgi complex for the synthesis of sphingomyelin (SM). <jats:italic>Chlamydia\u00a0trachomatis<\/jats:italic> has been suggested to employ CERT to produce SM in the inclusion by host SM synthases (SMSs). Here, we found that <jats:italic>C.\u00a0trachomatis<\/jats:italic> proliferates and produces infective progeny even in <jats:italic>SMS1<\/jats:italic> and <jats:italic>SMS2<\/jats:italic> double\u2010knockout HeLa cells, but not in the <jats:italic>SMS1<\/jats:italic>\/<jats:italic>SMS2<\/jats:italic>\/<jats:italic>CERT<\/jats:italic> triple\u2010knockout cells. Interestingly, infected cells convert ceramide to SM without host SMSs. These results suggest that <jats:italic>C.\u00a0trachomatis<\/jats:italic>\u2010infected cells can convert ceramide to SM without host SMSs after CERT\u2010mediated transfer of ceramide to the inclusions.<\/jats:p>","DOI":"10.1002\/1873-3468.13632","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T17:20:01Z","timestamp":1570641601000},"page":"519-529","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["<i>Chlamydia\u00a0trachomatis<\/i>\u2010infected human cells convert ceramide to sphingomyelin without sphingomyelin synthases 1 and 2"],"prefix":"10.1002","volume":"594","author":[{"given":"Yuriko","family":"Tachida","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Cell Biology National Institute of Infectious Diseases Shinjuku\u2010ku 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