{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T13:58:57Z","timestamp":1747144737440,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T00:00:00Z","timestamp":1574899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002301","name":"Eesti Teadusagentuur","doi-asserted-by":"publisher","award":["PRG057","PUT4"],"award-info":[{"award-number":["PRG057","PUT4"]}],"id":[{"id":"10.13039\/501100002301","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["1819"],"award-info":[{"award-number":["1819"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p><jats:italic>Lgr5\u2010LacZ<\/jats:italic> mice harbor the <jats:italic>Escherichia\u00a0coli LacZ<\/jats:italic> gene encoding \u03b2\u2010galactosidase (\u03b2\u2010gal) under the control of the <jats:italic>Lgr5<\/jats:italic> promoter, a stem\/progenitor cell marker. In injured livers of <jats:italic>Lgr5\u2010LacZ<\/jats:italic> mice, cells expressing \u03b2\u2010galactosidase (\u03b2\u2010gal) are considered as potential bipotent liver progenitors; however, their origin and identity remain unknown. Unexpectedly, using lineage tracing, we demonstrate that the \u03b2\u2010gal<jats:sup>+<\/jats:sup> cells do not originate from liver parenchymal cells. Instead, \u03b2\u2010gal<jats:sup>+<\/jats:sup> cells, isolated from injured livers of both <jats:italic>Lgr5\u2010LacZ<\/jats:italic> and wild\u2010type mice, are positive for markers of Kupffer cells, liver\u2010resident macrophages. The \u03b2\u2010gal expression in these cells is a result of elevated expression of the endogenous beta\u2010galactosidase Glb1. In injured livers of <jats:italic>Lgr5\u2010LacZ<\/jats:italic> mice, bacterial <jats:italic>\u03b2\u2010gal<\/jats:italic> expression is very low, suggesting transgene silencing. The gene expression profile of the \u03b2\u2010gal<jats:sup>+<\/jats:sup> Kupffer cells from injured livers suggests a role in liver regeneration.<\/jats:p>","DOI":"10.1002\/1873-3468.13669","type":"journal-article","created":{"date-parts":[[2019,11,9]],"date-time":"2019-11-09T18:02:30Z","timestamp":1573322550000},"page":"958-970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endogenous beta\u2010galactosidase activity marks a TREM2\u2010expressing Kupffer cell population in injured livers of <i>Lgr5\u2010LacZ<\/i> and wild\u2010type mice"],"prefix":"10.1002","volume":"594","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2457-5928","authenticated-orcid":false,"given":"Mariliis","family":"Klaas","sequence":"first","affiliation":[{"name":"Institute of Molecular and Cell Biology University of Tartu 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