{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:39:32Z","timestamp":1765805972193,"version":"3.40.5"},"reference-count":59,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T00:00:00Z","timestamp":1600214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>Among the two GroEL paralogs in <jats:italic>Mycobacterium\u00a0tuberculosis<\/jats:italic>, GroEL1 and GroEL2, GroEL1 has a characteristic histidine\u2010rich C terminus. Since histidine richness is likely to be involved in metal binding, we attempted to decipher the role of GroEL1 in chelating metals and the consequence on <jats:italic>M.\u00a0tuberculosis<\/jats:italic> physiology. Isothermal titration calorimetry showed that GroEL1 binds copper and other metals. Mycobacterial viability assay, redox balance, and DNA protection assay concluded that GroEL1 protects from copper stress <jats:italic>in\u00a0vitro<\/jats:italic>. Solution X\u2010ray scattering and constrained modeling of GroEL1 \u2212\/+ copper ions showed reorientation of the apical domain as seen in functional assembly. We conclude that the duplication of chaperonin genes in <jats:italic>M.\u00a0tuberculosis<\/jats:italic> might have led to their evolutionary divergence and consequent functional divergence of chaperonins.<\/jats:p>","DOI":"10.1002\/1873-3468.13906","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T01:15:16Z","timestamp":1598577316000},"page":"3305-3323","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A novel function of <i>Mycobacterium\u00a0tuberculosis<\/i> chaperonin paralog GroEL1 in copper homeostasis"],"prefix":"10.1002","volume":"594","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7383-7029","authenticated-orcid":false,"given":"Mohammed Yousuf","family":"Ansari","sequence":"first","affiliation":[{"name":"National Centre for Cell Science University of Pune Campus Pune India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakshi 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