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We know that both outer and inner membrane proteins FptA and FptX are involved in the ferri\u2010PCH uptake, but this process requires increasing molecular and biochemical knowledge. Here, using bacterial two\u2010hybrid and copurification assays we identified the fatty acid coenzyme\u2010A ligase FadD1 as a novel interacting partner of the inner membrane transporter FptX and found that FadD1 may play a role in PCH production. We managed to purify the FadD1\u2013FptX inner membrane complex and obtained low\u2010resolution 3D models, opening the way for future high\u2010resolution structures.<\/jats:p>","DOI":"10.1002\/1873-3468.14012","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T04:50:17Z","timestamp":1606884617000},"page":"370-378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of the fatty acid coenzyme\u2010A ligase FadD1 as an interacting partner of FptX in the <i>Pseudomonas\u00a0aeruginosa<\/i> pyochelin pathway"],"prefix":"10.1002","volume":"595","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9007-7014","authenticated-orcid":false,"given":"B\u00e9atrice","family":"Roche","sequence":"first","affiliation":[{"name":"UMR7242 ESBS CNRS Illkirch France"},{"name":"UMR7242 ESBS Universit\u00e9 de Strasbourg Illkirch France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ga\u00ebtan L. 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