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The major sperm protein (MSP) domain of VAP binds to a variety of sequences which are referred to as FFAT\u2010like motifs. In this study, we investigated the interactions of eight peptides containing FFAT\u2010like motifs with the VAP\u2010A MSP domain (VAP\u2010A<jats:sub>MSP<\/jats:sub>) by solution NMR. Six of eight peptides are specifically bound to VAP\u2010A. Furthermore, we found that the RNA\u2010dependent RNA polymerase of severe acute respiratory syndrome coronavirus 2 has an FFAT\u2010like motif which specifically binds to VAP\u2010A<jats:sub>MSP<\/jats:sub> as well as other FFAT\u2010like motifs. Our results will contribute to the discovery of new VAP interactors.<\/jats:p>","DOI":"10.1002\/1873-3468.14166","type":"journal-article","created":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T07:50:44Z","timestamp":1627372244000},"page":"2248-2256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Sequence requirements of the FFAT\u2010like motif for specific binding to VAP\u2010A are revealed by NMR"],"prefix":"10.1002","volume":"595","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9272-0026","authenticated-orcid":false,"given":"Kyoko","family":"Furuita","sequence":"first","affiliation":[{"name":"Institute for Protein Research Osaka University Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Hiraoka","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering Science Yokohama National University Yokohama 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