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We report a non\u2010negative blind deconvolution (NNBD) approach to quantify calcium (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) handling, beating force and contractility in human\u2010induced pluripotent stem cell\u2010derived cardiomyocytes (iPSC\u2010CMs) at the single\u2010cell level. We employed CRISPR\/Cas gene editing to introduce a dilated cardiomyopathy (DCM)\u2010causing mutation in troponin T (TnT), TnT\u2010R141W, into wild\u2010type control iPSCs (MUT). The NNDB\u2010based method enabled data parametrization, fitting and analysis in wild\u2010type controls versus isogenic MUT iPSC\u2010CMs. Of note, Cas9\u2010edited TnT\u2010R141W iPSC\u2010CMs revealed significantly reduced beating force and prolonged contractile event duration. The NNBD\u2010based platform provides an alternative framework for improved quantitation of molecular disease phenotypes and may contribute to the development of novel diagnostic tools.\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.14189","type":"journal-article","created":{"date-parts":[[2021,9,5]],"date-time":"2021-09-05T14:40:10Z","timestamp":1630852810000},"page":"2544-2557","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Non\u2010negative blind deconvolution for signal processing in a CRISPR\u2010edited iPSC\u2010cardiomyocyte model of dilated cardiomyopathy"],"prefix":"10.1002","volume":"595","author":[{"given":"Hang","family":"Xu","sequence":"first","affiliation":[{"name":"Heart Research Center Department of Cardiology and Pneumology University Medical Center Goettingen University Germany"},{"name":"DZHK (German Center for Cardiovascular Research) Partner Site Goettingen 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