{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:40:35Z","timestamp":1790671235172,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1T32GM133352\u201001A1"],"award-info":[{"award-number":["1T32GM133352\u201001A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5T32GM007105\u201044"],"award-info":[{"award-number":["5T32GM007105\u201044"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p><jats:italic>Chlamydia\u2009trachomatis<\/jats:italic>, an obligate intracellular bacterium with limited metabolic capabilities, possesses the futalosine pathway for menaquinone biosynthesis. Futalosine pathway enzymes have promise as narrow\u2010spectrum antibiotic targets, but the activity and essentiality of chlamydial menaquinone biosynthesis have yet to be established. In this work, menaquinone\u20107 (MK\u20107) was identified as a <jats:italic>C. trachomatis<\/jats:italic>\u2010produced quinone through liquid chromatography\u2010tandem mass spectrometry. An immunofluorescence\u2010based assay revealed that treatment of <jats:italic>C. trachomatis<\/jats:italic>\u2010infected HeLa cells with the futalosine pathway inhibitor docosahexaenoic acid (DHA) reduced inclusion number, inclusion size, and infectious progeny. Supplementation with MK\u20107 nanoparticles rescued the effect of DHA on inclusion number, indicating that the futalosine pathway is a target of DHA in this system. These results open the door for menaquinone biosynthesis inhibitors to be pursued in antichlamydial development.<\/jats:p>","DOI":"10.1002\/1873-3468.14223","type":"journal-article","created":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T14:54:45Z","timestamp":1636210485000},"page":"2995-3005","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of the futalosine pathway for menaquinone biosynthesis suppresses <i>Chlamydia trachomatis<\/i> infection"],"prefix":"10.1002","volume":"595","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7229-9644","authenticated-orcid":false,"given":"Brianne M.","family":"Dudiak","sequence":"first","affiliation":[{"name":"Department of Chemistry Duke University Durham NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tri M.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Chemistry Duke University Durham NC 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