{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:01:53Z","timestamp":1792062113796,"version":"4.4.0"},"reference-count":79,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,12]],"date-time":"2021-12-12T00:00:00Z","timestamp":1639267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF18OC0032242"],"award-info":[{"award-number":["NNF18OC0032242"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Monocopper lytic polysaccharide monooxygenases (LPMOs) catalyse oxidative cleavage of glycosidic bonds in a reductant\u2010dependent reaction. Recent studies indicate that LPMOs, rather than being O<jats:sub>2<\/jats:sub>\u2010dependent monooxygenases, are H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010dependent peroxygenases. Here, we describe <jats:italic>Ssc<\/jats:italic>LPMO10B, a novel LPMO from the phytopathogenic bacterium <jats:italic>Streptomyces scabies<\/jats:italic> and address links between this enzyme\u2019s catalytic rate and in\u2009situ hydrogen peroxide production in the presence of ascorbic acid, gallic acid and <jats:sc>l<\/jats:sc>\u2010cysteine. Studies of Avicel degradation showed a clear correlation between the catalytic rate of <jats:italic>Ssc<\/jats:italic>LPMO10B and the rate of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> generation in the reaction mixture. We also assessed the impact of oxidised ascorbic acid, dehydroascorbic acid (DHA), on LPMO activity, since DHA, which is not considered a reductant, was recently reported to drive LPMO reactions. Kinetic studies, combined with NMR analysis, showed that DHA is unstable and converts into multiple derivatives, some of which are redox active and can fuel the LPMO reaction by reducing the active site copper and promoting H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production. These results show that the apparent monooxygenase activity observed in <jats:italic>Ssc<\/jats:italic>LPMO10B reactions without exogenously added H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> reflects a peroxygenase reaction.<\/jats:p>","DOI":"10.1002\/1873-3468.14246","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T16:32:04Z","timestamp":1638289924000},"page":"53-70","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["The impact of reductants on the catalytic efficiency of a lytic polysaccharide monooxygenase and the special role of dehydroascorbic acid"],"prefix":"10.1002","volume":"596","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6754-5852","authenticated-orcid":false,"given":"Anton A.","family":"Stepnov","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science NMBU \u2010 Norwegian University of Life Sciences \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6229-2211","authenticated-orcid":false,"given":"Idd A.","family":"Christensen","sequence":"additional","affiliation":[{"name":"NOBIPOL Department of Biotechnology and Food Science NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8236-7986","authenticated-orcid":false,"given":"Zarah","family":"Forsberg","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science NMBU \u2010 Norwegian University of Life Sciences \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1613-4663","authenticated-orcid":false,"given":"Finn L.","family":"Aachmann","sequence":"additional","affiliation":[{"name":"NOBIPOL Department of Biotechnology and Food Science NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1644-3223","authenticated-orcid":false,"given":"Gaston","family":"Courtade","sequence":"additional","affiliation":[{"name":"NOBIPOL Department of Biotechnology and Food Science NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9220-8743","authenticated-orcid":false,"given":"Vincent G. H.","family":"Eijsink","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science NMBU \u2010 Norwegian University of Life Sciences \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1192231"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.689"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1105776108"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408090111"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2558"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-015-0284-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1323629111"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj1342"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402771111"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201800211"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb200351y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-015-9887-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504468200"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2016.07.012"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.602227"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2016.12.012"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20170549"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.004269"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02612-18"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-019-1548-y"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3043"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00156-18"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja210657t"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2470"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922499117"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00029-18"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15704"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-020-01673-4"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19561-8"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.817593"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1754-6834-5-79"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408115111"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1801153115"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2004277117"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.530196"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi5000433"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-6031(95)02373-A"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00088a002"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715769809069278"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715769900300571"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-021-01879-0"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.008196"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf3165"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-016-0594-y"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21473-0"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2009.00561.x"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2004.01203.x"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-021-01902-4"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3988"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky949"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.722447"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-019-1449-0"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-019-03135-0"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-019-1392-0"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1063-6"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-021-01894-1"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1199-4"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1904889117"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja1014103"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-405883-5.00002-8"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1453"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00968-X"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00166-7"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2017.03.006"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2019.108240"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b00778"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20180688"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955712798868986"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ers330"},{"key":"e_1_2_9_71_1","doi-asserted-by":"crossref","unstructured":"WoodTMWood TM. Preparation of crystalline amorphous and dyed cellulase substrates. Methods Enzymol. 1988;160:19\u201025.","DOI":"10.1016\/0076-6879(88)60103-0"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks479"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0036-z"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3529391"},{"key":"e_1_2_9_75_1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1385\/1-59259-890-0:571","volume-title":"Protein identification and analysis tools on the ExPASy server in The Proteomics Protocols Handbook","author":"Gasteiger E","year":"2005"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00089a006"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6GC01666A"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022836027055"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmr.a.20223"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-013-0503-3"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.14246","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1873-3468.14246","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.14246","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T05:55:58Z","timestamp":1692856558000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.14246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,12]]},"references-count":79,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/1873-3468.14246"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.14246","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,12]]},"assertion":[{"value":"2021-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}