{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:24:09Z","timestamp":1792268649493,"version":"4.4.0"},"reference-count":63,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T00:00:00Z","timestamp":1642723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["RGY0080\/2018"],"award-info":[{"award-number":["RGY0080\/2018"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["862137"],"award-info":[{"award-number":["862137"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:p>In autophagy, LC3\u2010positive autophagophores fuse and encapsulate the autophagic cargo in a double\u2010membrane structure. In contrast, lipidated LC3 (LC3\u2010II) is directly formed at the phagosomal membrane in LC3\u2010associated phagocytosis (LAP). In this study, we dissected the effects of autophagy inhibitors on LAP. SAR405, an inhibitor of VPS34, reduced levels of LC3\u2010II and inhibited LAP. In contrast, the inhibitors of endosomal acidification bafilomycin A1 and chloroquine increased levels of LC3\u2010II, due to reduced degradation in acidic lysosomes. However, while bafilomycin A1 inhibited LAP, chloroquine did not. Finally, EACC, which inhibits the fusion of autophagosomes with lysosomes, promoted LC3 degradation possibly by the proteasome. Targeting LAP with small molecule inhibitors is important given its emerging role in infectious and autoimmune diseases.<\/jats:p>","DOI":"10.1002\/1873-3468.14280","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T17:19:16Z","timestamp":1641835156000},"page":"491-509","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Novel and conventional inhibitors of canonical autophagy differently affect LC3\u2010associated phagocytosis"],"prefix":"10.1002","volume":"596","author":[{"given":"Femmy C.","family":"Stempels","sequence":"first","affiliation":[{"name":"Department of Molecular Immunology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen The\u2009Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maaike H.","family":"Janssens","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen The\u2009Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"ter Beest","sequence":"additional","affiliation":[{"name":"Department of Tumor Immunology Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The\u2009Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob J.","family":"Mesman","sequence":"additional","affiliation":[{"name":"Department of Microbiology RIBES Faculty of Science Radboud University Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia H.","family":"Revelo","sequence":"additional","affiliation":[{"name":"Department of Tumor Immunology Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The\u2009Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melina","family":"Ioannidis","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen The\u2009Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2180-6735","authenticated-orcid":false,"given":"Geert","family":"van den Bogaart","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen The\u2009Netherlands"},{"name":"Department of Tumor Immunology Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The\u2009Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/boc.201700028"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2019.04.016"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14354"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010070"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.03.020"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06421"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01397"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1113421108"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3192"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.01.018"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1569297"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21829-6"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201308173"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aan4144"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17950"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.08.061"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160170"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00279"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811045106"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.01.018"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e18\u201008\u20100482"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.11.001"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3066"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00037.2017"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1681"},{"key":"e_1_2_9_27_1","volume-title":"Guidelines for the use and interpretation of assays for monitoring autophagy","author":"Klionsky DJ","year":"2021"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-2104-y"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2018.12.015"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202009128"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.4161\/15548627.2014.984277"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2017.1287653"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.90.3.665"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2018.1474314"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1006-9"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjy038"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.108.149336"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2012.03.003"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10477"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32256-x"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-3701-1-10"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-14221-2"},{"key":"e_1_2_9_44_1","article-title":"V\u2010ATPase is a universal regulator of LC3 associated phagocytosis and non\u2010canonical autophagy","author":"Hooper KM","year":"2021","journal-title":"BioRxiv"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.196931"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.7.8.15597"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20200835"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.056"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.1.2.1697"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.09.022"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfr179"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-09-0704"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50034"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11320"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20110006"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.05.035"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.26237"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv018"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00701.x"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1066957"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8007"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.22"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.117.6.2233"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.7972"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.14280","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1873-3468.14280","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.14280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T15:25:43Z","timestamp":1693149943000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.14280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,21]]},"references-count":63,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/1873-3468.14280"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.14280","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,21]]},"assertion":[{"value":"2021-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}