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The reported arylalkylamine substrates are all primary amines. In this study, we report a novel substrate, a secondary amine, of <jats:italic>Ae<\/jats:italic>.\u2009<jats:italic>aegypti<\/jats:italic> aaNAT1. The recombinant aaNAT1 protein exhibited high activity to a secondary amine, epinephrine, which has not been reported for any aaNATs previously. Structure\u2013activity relationship study demonstrated that aaNAT1 has an epinephrine\u2010binding site, and molecular docking and dynamic simulation showed that epinephrine is quite stable in the active cavity. Further functional studies demonstrated that epinephrine affected mosquito fecundity, egg hatching and development. The new biochemical function of aaNAT1 in metabolizing epinephrine could reduce some negative effects of the compound in the mosquito.<\/jats:p>","DOI":"10.1002\/1873-3468.14316","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T08:25:03Z","timestamp":1645172703000},"page":"1081-1091","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Arylalkalamine <i>N<\/i>\u2010acetyltransferase\u20101 acts on a secondary amine in the yellow fever mosquito, <i>Aedes aegypti<\/i>"],"prefix":"10.1002","volume":"596","author":[{"given":"Lei","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Tropical Veterinary Medicine and Vector Biology School of Life Sciences Hainan University  Haikou China"},{"name":"One Health Institute Hainan University  Haikou 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