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Whether such heteromers are expressed in human vascular smooth muscle cells (hVSMCs) is unknown. Bioluminescence resonance energy transfer confirmed formation of recombinant CCR2:\u03b1<jats:sub>1b<\/jats:sub>\u2010AR heteromers. Proximity ligation assays detected CCR2:\u03b1<jats:sub>1B<\/jats:sub>\u2010AR heteromers in hVSMCs and human mesenteric arteries. CCR2:\u03b1<jats:sub>1B<\/jats:sub>\u2010AR heteromerization <jats:italic>per\u2009se<\/jats:italic> enhanced \u03b1<jats:sub>1B<\/jats:sub>\u2010AR\u2010mediated G\u03b1<jats:sub>q<\/jats:sub>\u2010coupling. Chemokine (C\u2010C motif) ligand 2 (CCL2) binding to CCR2 inhibited G\u03b1<jats:sub>q<\/jats:sub> activation <jats:italic>via<\/jats:italic> \u03b1<jats:sub>1B<\/jats:sub>\u2010AR, cross\u2010recruited \u03b2\u2010arrestin to and induced internalization of \u03b1<jats:sub>1B<\/jats:sub>\u2010AR in recombinant systems and in hVSMCs. Our findings suggest that CCR2 within CCR2:\u03b1<jats:sub>1B<\/jats:sub>\u2010AR heteromers biases \u03b1<jats:sub>1B<\/jats:sub>\u2010AR signaling and provide a mechanism for previous observations suggesting a role for CCL2\/CCR2 in the regulation of cardiovascular function.<\/jats:p>","DOI":"10.1002\/1873-3468.14463","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T08:19:10Z","timestamp":1659514750000},"page":"2706-2716","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Heteromerization between <scp>\u03b1<sub>1B<\/sub><\/scp>\u2010adrenoceptor and chemokine (<scp>C\u2010C<\/scp> motif) receptor 2 biases <scp>\u03b1<sub>1B<\/sub><\/scp>\u2010adrenoceptor signaling: Implications for vascular function"],"prefix":"10.1002","volume":"596","author":[{"given":"Xianlong","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Surgery, Morsani College of Medicine 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