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The proline\u2010rich domain (PRD) of WASp contributes to branching nucleation, and the PRD of budding yeast Las17 binds actin filaments [Urbanek AN et\u00a0al. (2013) <jats:italic>Curr Biol<\/jats:italic> 23, 196\u2013203]. Biochemical assays showed the recombinant PRD of fission yeast <jats:italic>Schizosaccharomyces pombe<\/jats:italic> Wsp1p binds actin filaments with micromolar affinity. Recombinant PRDs of both Wsp1p and Las17p slowed the elongation of actin filaments by Mg\u2010ATP\u2010actin monomers by half and slowed the spontaneous polymerization of Mg\u2010ATP\u2010actin monomers modestly. The affinity of PRDs of WASp\u2010family proteins for actin filaments is high enough to contribute to the reported stimulation of actin filament branching by Arp2\/3 complex.<\/jats:p>","DOI":"10.1002\/1873-3468.14571","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T07:25:11Z","timestamp":1674026711000},"page":"672-681","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The proline\u2010rich domain of fission yeast <scp>WASp<\/scp> (Wsp1p) interacts with actin filaments and inhibits actin polymerization"],"prefix":"10.1002","volume":"597","author":[{"given":"Aaron D.","family":"Rosenbloom","sequence":"first","affiliation":[{"name":"Department of Chemistry Yale University  New Haven CT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1785-2969","authenticated-orcid":false,"given":"Thomas 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