{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:14:57Z","timestamp":1786976097165,"version":"build-2736575974"},"reference-count":68,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:00:00Z","timestamp":1695513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008655","name":"Hamaguchi Foundation for the Advancement of Biochemistry","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003806","name":"Hokuto Foundation for Bioscience","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003806","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008695","name":"Japan Foundation for Applied Enzymology","doi-asserted-by":"publisher","award":["2023\u201010"],"award-info":[{"award-number":["2023\u201010"]}],"id":[{"id":"10.13039\/100008695","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007807","name":"Japan Science Society","doi-asserted-by":"publisher","award":["2023\u20104003"],"award-info":[{"award-number":["2023\u20104003"]}],"id":[{"id":"10.13039\/501100007807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17H03650"],"award-info":[{"award-number":["JP17H03650"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18J20003"],"award-info":[{"award-number":["JP18J20003"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20H03205"],"award-info":[{"award-number":["JP20H03205"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21J00197"],"award-info":[{"award-number":["JP21J00197"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K15054"],"award-info":[{"award-number":["JP22K15054"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K19747"],"award-info":[{"award-number":["JP22K19747"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22KK0251"],"award-info":[{"award-number":["JP22KK0251"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011907","name":"Mishima Kaiun Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011907","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008664","name":"Ono Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008664","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008608","name":"Sumitomo Foundation","doi-asserted-by":"publisher","award":["2200431"],"award-info":[{"award-number":["2200431"]}],"id":[{"id":"10.13039\/100008608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007434","name":"Suzuken Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007434","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006089","name":"Tojuro Iijima Foundation for Food Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017421","name":"Toyo Suisan Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:p>\n                    Sphingomyelin (SM) synthase 1 (SMS1), which is involved in lipodystrophy, deafness, and thrombasthenia, generates diacylglycerol (DG) and SM using phosphatidylcholine (PC) and ceramide as substrates. Here, we found that SMS1 possesses DG\u2010generating activities\n                    <jats:italic>via<\/jats:italic>\n                    hydrolysis of PC and phosphatidylethanolamine (PE) in the absence of ceramide and ceramide phosphoethanolamine synthase (CPES) activity. In the presence of the same concentration (4.7\u2009mol%) of PC and ceramide, the amounts of DG produced by SMS and PC\u2010phospholipase C (PLC) activities of SMS1 were approximately 65% and 35% of total DG production, respectively. PC\u2010PLC activity showed substrate selectivity for saturated and\/or monounsaturated fatty acid\u2010containing PC species. A PC\u2010PLC\/SMS inhibitor, D609, inhibited only SMS activity. Mn\n                    <jats:sup>2+<\/jats:sup>\n                    inhibited only PC\u2010PLC activity. Intriguingly, DG attenuated SMS\/CPES activities. Our study indicates that SMS1 is a unique enzyme with PC\u2010PLC\/PE\u2010PLC\/SMS\/CPES activities.\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.14735","type":"journal-article","created":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T11:38:22Z","timestamp":1694864302000},"page":"2672-2686","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Human sphingomyelin synthase 1 generates diacylglycerol in the presence and absence of ceramide\n                    <i>via<\/i>\n                    multiple enzymatic activities"],"prefix":"10.1002","volume":"597","author":[{"given":"Rika","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Chemistry, Graduate School of Science Chiba University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3645-8382","authenticated-orcid":false,"given":"Chiaki","family":"Murakami","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate School of Science Chiba University  Japan"},{"name":"Institute for Advanced Academic Research Chiba University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3349-7899","authenticated-orcid":false,"given":"Kamila","family":"Dilimulati","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate School of Science Chiba University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Atsuta\u2010Tsunoda","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate School of Science Chiba University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuma","family":"Kawai","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate School of Science Chiba University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0857-0377","authenticated-orcid":false,"given":"Fumio","family":"Sakane","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate School of Science Chiba University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.12.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.12997\/jla.2020.9.3.380"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401205200"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600034"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200903152"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.33594\/000000200"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.179176"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00272.2015"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-04-491290"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0164298"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061380"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxs008"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxy082"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2019.06.038"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37829"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322509"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advenzreg.2006.01.005"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20071040"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2007.04.006"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2009.01.010"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2017.09.003"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/138945008785132394"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2016.00082"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.14.8394"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604104103"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.035"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702481200"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.590950"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-017-4366-4"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.02.165"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2019.158608"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13765"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.012369"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100454"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(80)91227-9"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb15972.x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb17311.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(66)90104-4"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-030212-183750"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.101398"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.13628"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.07.024"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.03.010"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.20.11872"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.191783"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)40687-X"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.32.18711"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2015.04.003"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-011-0659-z"},{"key":"e_1_2_9_52_1","first-page":"287","article-title":"The antiviral, antitumoural xanthate D609 is a competitive inhibitor of phosphatidylcholine\u2010specific phospholipase C","volume":"22","author":"Amtmann E","year":"1996","journal-title":"Drugs Exp Clin Res"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2007.05.007"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(91)90002-Y"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(72)85431-6"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M059972"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13511"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20200389"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaa0974"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2016.10.004"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.16.7289-7302.2005"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.44"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2023.159307"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21186794"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2020.158630"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M076133"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M900230-JLR200"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M054627"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2200345"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.14735","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T06:48:29Z","timestamp":1764485309000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.14735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,24]]},"references-count":68,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1002\/1873-3468.14735"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.14735","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,24]]},"assertion":[{"value":"2023-06-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}