{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:08:44Z","timestamp":1793149724271,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HO2515\/11\u20101"],"award-info":[{"award-number":["HO2515\/11\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["I\u20105192"],"award-info":[{"award-number":["I\u20105192"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:p>\n                    Psilocybin, the natural hallucinogen from\n                    <jats:italic>Psilocybe<\/jats:italic>\n                    (magic) mushrooms, is a highly promising drug candidate for the treatment of depression and several other mental health conditions. Biosynthesis of psilocybin from the amino acid\n                    <jats:sc>l\u2010<\/jats:sc>\n                    tryptophan involves four strictly sequential modifications. The third of these, ATP\u2010dependent phosphorylation of the intermediate 4\u2010hydroxytryptamine, is catalysed by PsiK. Here we present a crystallographic analysis and a structure\u2010based mutagenesis study of this kinase, providing insight into its mode of substrate recognition. The results of our work will support future bioengineering efforts aimed at generating variants of psilocybin with enhanced therapeutic properties.\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.15042","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T00:39:03Z","timestamp":1729816743000},"page":"447-455","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Substrate recognition by the 4\u2010hydroxytryptamine kinase\n                    <scp>PsiK in<\/scp>\n                    psilocybin biosynthesis"],"prefix":"10.1002","volume":"599","author":[{"given":"Kai","family":"Rogge","sequence":"first","affiliation":[{"name":"Institute of Pharmacy Friedrich Schiller University  Jena Germany"},{"name":"Research Group Pharmaceutical Microbiology, Leibniz Institute of Natural Product Research and Infection Biology Hans Kn\u00f6ll Institute  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias Johannes","family":"Wagner","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy Friedrich Schiller University  Jena Germany"},{"name":"Research Group Pharmaceutical Microbiology, Leibniz Institute of Natural Product Research and Infection Biology Hans Kn\u00f6ll Institute  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Hoffmeister","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy Friedrich Schiller University  Jena Germany"},{"name":"Research Group Pharmaceutical Microbiology, Leibniz Institute of Natural Product Research and Infection Biology Hans Kn\u00f6ll Institute  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3300-6965","authenticated-orcid":false,"given":"Bernhard","family":"Rupp","sequence":"additional","affiliation":[{"name":"Department of General, Inorganic and Theoretical Chemistry University of Innsbruck  Austria"},{"name":"k.\u2010k. 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