{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:50:37Z","timestamp":1791517837805,"version":"4.3.4"},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM136213"],"award-info":[{"award-number":["R35GM136213"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:p>\n                    Translation terminates at UAG (amber), UGA (opal), and UAA (ochre) stop codons. In nature, readthrough of stop codons can be substantially enhanced by suppressor tRNAs. Stop\u2010codon suppression also provides powerful tools in synthetic biology and disease treatment. How stop\u2010codon suppression affects bacterial pathogenesis is poorly understood. Here, we show that suppression of UAG codons, but not UGA or UAA codons, attenuates expression of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    Pathogenicity Island 1 (SPI\u20101) genes, which are required for virulence. Consistently, amber suppression abolishes\n                    <jats:italic>Salmonella<\/jats:italic>\n                    infection of macrophages. Systematic genetic and biochemical analyses further show that amber suppression decreases the activity, but not the level, of the master SPI\u20101 regulator HilD. Our work thus demonstrates an unexpected selectivity of stop codons in regulating\n                    <jats:italic>Salmonella<\/jats:italic>\n                    virulence.\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.15075","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T13:54:10Z","timestamp":1734011650000},"page":"476-487","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Suppression of amber stop codons impairs pathogenicity in\n                    <i>Salmonella<\/i>"],"prefix":"10.1002","volume":"599","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8781-5983","authenticated-orcid":false,"given":"Zhihui","family":"Lyu","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics The University of Maryland  College Park MD 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