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Herein, we show that the known metalloenzyme inhibitor cyanide inhibits reductive activation of LPMOs by binding to the LPMO\u2010Cu(II) state and that the degree of inhibition depends on the concentrations of the polysaccharide substrate, the reductant and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    . Importantly, this analysis revealed differences between fungal\n                    <jats:italic>Nc<\/jats:italic>\n                    AA9C and bacterial\n                    <jats:italic>Sm<\/jats:italic>\n                    AA10A, which have different secondary copper coordination spheres. These differences were also highlighted by the observation that phosphate, a commonly used buffer ion, strongly inhibits\n                    <jats:italic>Nc<\/jats:italic>\n                    AA9C while not affecting reactions with\n                    <jats:italic>Sm<\/jats:italic>\n                    AA10A. The results provide insight into LPMO inhibition and catalysis and highlight pitfalls in the analysis thereof.\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.15105","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T06:17:22Z","timestamp":1738822642000},"page":"1317-1336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Functional variation among\n                    <scp>LPMOs<\/scp>\n                    revealed by the inhibitory effects of cyanide and buffer ions"],"prefix":"10.1002","volume":"599","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0321-4835","authenticated-orcid":false,"given":"Ole","family":"Golten","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenz","family":"Schwaiger","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Institute of Food Technology University of Natural Resources and Life Sciences  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8236-7986","authenticated-orcid":false,"given":"Zarah","family":"Forsberg","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsi R.","family":"Hall","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"},{"name":"School of Biological Sciences University of Canterbury  Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6754-5852","authenticated-orcid":false,"given":"Anton A.","family":"Stepnov","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom Z.","family":"Emrich\u2010Mills","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n","family":"Ayuso\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"},{"name":"Biotechnology Department Margarita Salas Center for Biological Research (CIB\u2010CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7259-6710","authenticated-orcid":false,"given":"Morten","family":"S\u00f8rlie","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5058-5874","authenticated-orcid":false,"given":"Roland","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Institute of Food Technology University of Natural Resources and Life Sciences  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5smund Kjendseth","family":"R\u00f8hr","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences (NMBU)  \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent G. 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