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Although ethylene glycol\u2010bis(\u03b2\u2010aminoethyl ether)\u2010N,N,N\u2032,N\u2032\u2010tetraacetic acid (EGTA E3889, Sigma\u2010Aldrich, CABA\u2010Argentina), a calcium chelator, is widely used to disrupt epithelial monolayers\n                    <jats:italic>in vitro<\/jats:italic>\n                    , its application to zebrafish\n                    <jats:italic>in vivo<\/jats:italic>\n                    remains limited. We found that short\u2010term exposure to EGTA induced tail damage ranging from minor and reversible to severe and lethal; at the tissue level, it caused disruption of cadherin\u2010mediated cell\u2013cell contacts and of the barrier function in the epidermis. Labeling with fluorescent quantum dots (QDs) enabled rapid characterization of damage severity, evidencing the disappearance of actin microridges in the enveloping layer. 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