{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T05:35:01Z","timestamp":1773120901252,"version":"3.50.1"},"reference-count":68,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/PR40174\/BTIS\/137\/45\/2022"],"award-info":[{"award-number":["BT\/PR40174\/BTIS\/137\/45\/2022"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Vesicle fusion events are crucial for the survival of\n                      <jats:italic>Giardia lamblia<\/jats:italic>\n                      as they drive nutrient uptake and morphological stage transitions. Unlike most eukaryotes,\n                      <jats:italic>Giardia<\/jats:italic>\n                      has a minimal vesicular trafficking machinery. We report a rare exception to this minimalism wherein two paralogues of N\u2010ethylmaleimide\u2010sensitive factor (NSF) are present in this parasite. Localization studies indicate that these highly homologous paralogues\u2014GlNSF\n                      <jats:sub>112681<\/jats:sub>\n                      and GlNSF\n                      <jats:sub>114776<\/jats:sub>\n                      \u2014likely function independently under various stress conditions, as GlNSF\n                      <jats:sub>112681<\/jats:sub>\n                      remains at peripheral vesicles, while the major pool of GlNSF\n                      <jats:sub>114776<\/jats:sub>\n                      redistributes to anterior flagella\u2010associated structures. These paralogues also exhibit selective affinity for the \u03b1\u2010soluble NSF attachment proteins (Gl\u03b1\u2010SNAPs). This selectivity stems from sequence divergences near their N termini. The two GlNSFs colocalize and coimmunoprecipitate, indicating the presence of a heterohexameric 20S complex in trophozoites. This study is the first to report the presence of a heterohexameric 20S complex and reveals adaptive specialization of vesicle trafficking machinery within a reduced eukaryotic system.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"box\" position=\"anchor\">\n                        <jats:caption>\n                          <jats:title>Impact statement<\/jats:title>\n                        <\/jats:caption>\n                        <jats:p>\n                          Here we report that a unicellular parasitic protist,\n                          <jats:italic>Giardia lamblia<\/jats:italic>\n                          , has two NSF paralogues, which is a rarity in eukaryotes. Although they share a high degree of homology, they are likely to discharge independent functions, especially under stress conditions.\n                        <\/jats:p>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/1873-3468.70225","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T05:11:02Z","timestamp":1763442662000},"page":"612-630","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The minimal vesicular trafficking machinery of\n                    <i>Giardia<\/i>\n                    has two\n                    <scp>NSF<\/scp>\n                    paralogues"],"prefix":"10.1002","volume":"600","author":[{"given":"Trisha","family":"Ghosh","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Bose Institute  Kolkata 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