{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:02:37Z","timestamp":1790600557993,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H02425"],"award-info":[{"award-number":["23H02425"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K05147"],"award-info":[{"award-number":["23K05147"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>Fused in sarcoma (FUS) forms phase\u2010separated condensates implicated in amyotrophic lateral sclerosis (ALS). Although millimolar ATP concentrations paradoxically dissolve FUS condensates through hydrotropic activity, condensates nevertheless persist in cells, suggesting active regulatory mechanisms. Here, using a reconstituted system, we show that the AAA+ATPase valosin\u2010containing protein (VCP) counteracts ATP\u2010driven dissolution of FUS condensates. VCP preserved both wild\u2010type and ALS\u2010linked P525L condensates under high ATP conditions, and this protection required catalytic ATPase activity rather than stable partitioning into condensates. The effect was abolished by the D2\u2010specific inhibitor ML240. Our findings establish direct biochemical evidence that VCP ATPase activity maintains FUS condensates under high ATP conditions, highlighting ATPase\u2010driven enzymatic control of liquid\u2013liquid phase separation as a potential general principle with implications for neurodegeneration.<\/jats:p>","DOI":"10.1002\/1873-3468.70328","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T05:49:53Z","timestamp":1773726593000},"page":"2787-2799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Valosin\u2010containing protein counteracts\n                    <scp>ATP<\/scp>\n                    \u2010driven dissolution of\n                    <scp>FUS<\/scp>\n                    condensates through its\n                    <scp>ATPase<\/scp>\n                    activity\n                    <i>in vitro<\/i>"],"prefix":"10.1002","volume":"600","author":[{"given":"Hitomi","family":"Kimura","sequence":"first","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University  Japan"},{"name":"WPI\u2010SKCM Hiroshima University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5556-7712","authenticated-orcid":false,"given":"Shin\u2010ichi","family":"Tate","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University  Japan"},{"name":"WPI\u2010SKCM Hiroshima University  Japan"},{"name":"Research Center for the Mathematics on Chromatin Live Dynamics (RcMcD) Hiroshima University  Japan"},{"name":"Institute of Advanced Mathematical Sciences (MIMS) Meiji University  Tokyo Japan"},{"name":"Graduate School of Medical Life Science Yokohama City University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8352-631X","authenticated-orcid":false,"given":"Kyota","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University  Japan"},{"name":"WPI\u2010SKCM Hiroshima University  Japan"},{"name":"Research Center for the Mathematics on Chromatin Live Dynamics (RcMcD) Hiroshima University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.7"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.11.054"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1134108"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166066"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3230"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.09.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.09.006"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.07.047"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165942"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.143"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.10.030"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.516492"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf6846"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.26335"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.11.180"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000327"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.09.014"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-019-0463-x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2025.169295"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)00143-X"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.11.036"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.14353"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201306058"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201608022"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201200520"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1313"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.12.032"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrp.2021.100343"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.11.020"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200908115"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.006"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.01.060"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01346"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E24-01-0013"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105585"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.093831"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-018-0085-x"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408695200"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202201027"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5894-09.2010"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.70328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1873-3468.70328","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.70328","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T12:53:59Z","timestamp":1790600039000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.70328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,16]]},"references-count":42,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/1873-3468.70328"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.70328","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/1873-3468.70328\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1873-3468.70328\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1873-3468.70328\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1873-3468.70328\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1873-3468.70328\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1873-3468.70328\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,16]]},"assertion":[{"value":"2026-01-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}