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Here, we demonstrate that both mRNA and protein levels of 14\u20103\u20103 zeta are markedly reduced in this mutant, while knockdown of 14\u20103\u20103 zeta in wild\u2010type CHO cells confers partial resistance to exogenous PS. Consistently, these knockdown cells display defective transbilayer movement of 1\u2010palmitoyl\u20102\u2010{6\u2010[(7\u2010nitro\u20101,3\u2010benz\u20102\u2010oxadiazol\u20104\u2010yl) amino] hexanoyl}\u2010\n                    <jats:italic>sn<\/jats:italic>\n                    \u2010glycero\u20103\u2010phosphoserine (C\n                    <jats:sub>6<\/jats:sub>\n                    \u2010NBD\u2010PS). However, 14\u20103\u20103 zeta knockdown does not further enhance PS resistance in ATP11C\u2010deficient cells. These findings indicate that 14\u20103\u20103 zeta plays a regulatory role in ATP11C\u2010dependent transbilayer movement of PS at the plasma membrane.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"box\" position=\"anchor\">\n                      <jats:caption>\n                        <jats:title>Impact statement<\/jats:title>\n                      <\/jats:caption>\n                      <jats:p>14\u20103\u20103\u03b6 regulates ATP11C\u2010dependent phosphatidylserine (PS) transbilayer movement at the plasma membrane. Reduced 14\u20103\u20103\u03b6 impairs PS flipping and confers resistance to exogenous PS in CHO cells, identifying 14\u20103\u20103\u03b6 as\u00a0a key modulator of phospholipid asymmetry and lipid\u2010induced cytotoxicity.<\/jats:p>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.70338","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T05:31:03Z","timestamp":1776317463000},"page":"3041-3050","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An isoform of 14\u20103\u20103 protein regulates transbilayer lipid movement at the plasma membrane"],"prefix":"10.1002","volume":"600","author":[{"given":"Akiko","family":"Yamaji\u2010Hasegawa","sequence":"first","affiliation":[{"name":"Lipid Biology Laboratory RIKEN  Saitama Japan"},{"name":"Laboratory for Neural Cell Dynamics, CBS, RIKEN  Saitama 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