{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:26:00Z","timestamp":1787127960359,"version":"build-2736575974"},"reference-count":100,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100024870","name":"Exacte en Natuurwetenschappen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012623","name":"Federation of European Biochemical Societies","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012623","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"abstract":"<jats:p>Single\u2010molecule biophysical techniques enable the study of biological systems by directly observing individual molecules and events, rather than measuring population averages. These approaches reveal transient states, rare events, conformational dynamics, and subpopulations that are often obscured in biochemical assays. The application of single\u2010molecule methods has been limited by technical complexity and the need for specialized instrumentation. Recent standardization of protocols, methodologies, and platforms has lowered these barriers, enabling broader adoption and promoting interdisciplinary collaborations that facilitate integration into biochemical research. In this review, we discuss how ensemble biochemistry and single\u2010molecule approaches are complementary, outline commonly used single\u2010molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double\u2010strand break repair.<\/jats:p>","DOI":"10.1002\/1873-3468.70444","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:50:08Z","timestamp":1787125808000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic perspectives\u2014How single\u2010molecule biophysics complement biochemical understanding"],"prefix":"10.1002","author":[{"given":"Sara","family":"De Bragan\u00e7a","sequence":"first","affiliation":[{"name":"Department of Cell Biology Radboud Institute for Molecular Life Sciences, Radboud University  Nijmegen The 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