{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:02:05Z","timestamp":1790157725075,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"abstract":"<jats:p>\n                    A prominent bacterial family in the human gut is\n                    <jats:italic>Bacteroidetes<\/jats:italic>\n                    , for which cobalamin (vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    ) is an essential micronutrient. Here, we characterized the structural\u2010function relationships underlying the role of three BtuG family proteins, BtuG1, BtuG2, and BtuG3, in cobalamin acquisition. We solved crystal structures (resolution between 2 and 1.5\u2009\u00c5) of these homologs bound to different cobalamin forms and the precursor cobinamide, and measured the binding affinities by grating coupled interferometry. We observed high binding affinities, with K\n                    <jats:sub>D<\/jats:sub>\n                    values in the low picomolar range, underlining their role in cobalamin scavenging. Mutations based on the structures resulted in altered binding affinities for cobalamin and its precursor. This study provides structural and quantitative insights into the B\n                    <jats:sub>12<\/jats:sub>\n                    scavenging mechanism of BtuG proteins.\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.70452","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:08:01Z","timestamp":1790154481000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural and biochemical analysis of a\n                    <scp>\n                      B\n                      <sub>12<\/sub>\n                    <\/scp>\n                    superbinder"],"prefix":"10.1002","author":[{"given":"Jose M.","family":"Martinez Felices","sequence":"first","affiliation":[{"name":"University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)  The\u00a0Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8966-111X","authenticated-orcid":false,"given":"Jacob J.","family":"Whittaker","sequence":"additional","affiliation":[{"name":"University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)  The\u00a0Netherlands"},{"name":"Department of Molecular Sciences Swedish University of Agricultural Sciences  Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5804-9689","authenticated-orcid":false,"given":"Dirk J.","family":"Slotboom","sequence":"additional","affiliation":[{"name":"University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)  The\u00a0Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2340-2216","authenticated-orcid":false,"given":"Albert","family":"Guskov","sequence":"additional","affiliation":[{"name":"University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)  The\u00a0Netherlands"},{"name":"Department of Biological Sciences, College of Medicine and Health Sciences Khalifa University  Abu Dhabi United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PBIO.1002533"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELL.2012.01.035"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41579\u2010018\u20100036\u2010X"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.37138"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11234"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.COPBIO.2012.08.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/FGENE.2015.00148"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MIB.2015.06.014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00253\u2010001\u20100902\u20107"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.12.007"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010017\u20100631\u2010y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/AJCN\/87.5.1324"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3945\/AJCN.111.013441"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.161.3.896\u2010903.1985"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.3.706\u2010717.2002"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/BS.VH.2022.01.008"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010biochem\u2010011520\u2010105201"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201005441\u20109"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00008\u201007"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/JEX2.61"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201040427\u20102"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"XDS","volume":"66","author":"Kabsch W","year":"2010","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003819\u20102"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2053273315099672"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911001314"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02845\u201021"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.20.023162"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/JR9650006485"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41575"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/SCIENCE.2911722"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41594\u2010020\u20100469\u20106"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099\u20101352(199903\/04)12:2<131::AID\u2010JMR454>3.0.CO;2\u2010M"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/EMBOJ.2010.123"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/NATURE07456"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/PROTEIN\/GZN028"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1856"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.70452","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1873-3468.70452","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1873-3468.70452","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:08:02Z","timestamp":1790154482000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1873-3468.70452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"references-count":38,"alternative-id":["10.1002\/1873-3468.70452"],"URL":"https:\/\/doi.org\/10.1002\/1873-3468.70452","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"assertion":[{"value":"2026-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1873-3468.70452"}}