{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:04:17Z","timestamp":1790661857814,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004785","name":"NordForsk","doi-asserted-by":"publisher","award":["82845"],"award-info":[{"award-number":["82845"]}],"id":[{"id":"10.13039\/501100004785","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"Svenska Forskningsr\u00e5det Formas","doi-asserted-by":"publisher","award":["2016\u201001325"],"award-info":[{"award-number":["2016\u201001325"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"abstract":"<jats:p>\n                    Cyanobacteria are promising microbial hosts for production of succinate, a central metabolite of the tricarboxylic acid (TCA) cycle, which cyanobacteria can secrete during anoxic fermentation. To investigate the route for succinate formation, we generated strains of\n                    <jats:italic>Synechocystis<\/jats:italic>\n                    PCC6803 lacking subunits of the succinate dehydrogenase complex (SDH). During dark anaerobic fermentation, the deletion strains show higher succinate accumulation than the wild type, indicating succinate dehydrogenase activity of SDH rather than fumarate reduction. Another potential route for succinate formation from fumarate is as a byproduct of the L\u2010aspartate oxidase (Laspo) reaction. Using purified\n                    <jats:italic>Syn<\/jats:italic>\n                    Laspo, we show that in addition to L\u2010aspartate oxidation, the enzyme exhibits L\u2010aspartate\u2010fumarate oxidoreductase activity, generating succinate. This work contributes to the understanding of the cyanobacterial TCA cycle.\n                  <\/jats:p>","DOI":"10.1002\/1873-3468.70460","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:04:04Z","timestamp":1789470244000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["L\u2010aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in\n                    <i>Synechocystis<\/i>\n                    sp.\u00a0\n                    <scp>PCC6803<\/scp>"],"prefix":"10.1002","author":[{"given":"Kateryna","family":"Kukil","sequence":"first","affiliation":[{"name":"Microbial Chemistry, Department of Chemistry \u2013 \u00c5ngstr\u00f6m Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A.","family":"Hawkes","sequence":"additional","affiliation":[{"name":"Analytical Chemistry, Department of Chemistry for Life Sciences Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5740-926X","authenticated-orcid":false,"given":"Cecilia","family":"Blikstad","sequence":"additional","affiliation":[{"name":"Microbial Chemistry, Department of Chemistry \u2013 \u00c5ngstr\u00f6m Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6413-1443","authenticated-orcid":false,"given":"Pia","family":"Lindberg","sequence":"additional","affiliation":[{"name":"Microbial Chemistry, Department of Chemistry \u2013 \u00c5ngstr\u00f6m Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bbb.105"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.04.006"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2019.101645"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.202000127"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo10060250"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-020-01727-7"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2016.10.014"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meteno.2016.04.003"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2018.05.013"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01064"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32354"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2021.03.007"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12699"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1210858"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26287"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.14.4251-4258.2001"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.3.714-722.2000"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01972"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-021-01529-y"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0427u.x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.8.3012-3023.2006"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07059.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcx162"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2016.06.026"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00862-10"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1997.tb00350.x"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.31.100177.001301"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.5b00070"},{"key":"e_1_2_10_30_1","first-page":"539","volume-title":"Methods in Enzymology","author":"Heidorn T","year":"2011"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204273"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06641.x"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi015939r"},{"key":"e_1_2_10_34_1","article-title":"Regulation of l\u2010aspartate oxidase contributes to NADP+ biosynthesis in Synechocystis sp. 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