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This is particularly the case for high\u2010grade serous adenocarcinomas of the ovary and peritoneum, which are commonly diagnosed at an advanced stage, and collectively treated and managed similarly. We characterized the <jats:italic>N<\/jats:italic>\u2010 and <jats:italic>O<\/jats:italic>\u2010glycome of serous ovarian (<jats:styled-content style=\"fixed-case\">OC<\/jats:styled-content>) and peritoneal cancer (<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>) tissues using <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ESI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> profiling and validated the discriminatory glycans and their corresponding glyco\u2010gene expression levels using cell lines and transcriptomic data from 232 patients. Overall, the <jats:italic>N<\/jats:italic>\u2010 and <jats:italic>O<\/jats:italic>\u2010glycan repertoires of both cancer types were found to comprise mostly of \u03b12,6\u2010sialylated glycan structures, with the majority of <jats:italic>N<\/jats:italic>\u2010glycans displaying the biantennary mono\u2010 and disialylation as well as bisecting\u2010type biantennary glycans. The <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> profiling by <jats:styled-content style=\"fixed-case\">PGC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> also revealed several glycan structural isomers that corresponded to Lacdi<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>c\u2010type (Gal<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>c\u03b21\u20104Glc<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>c) motifs that were unique to the serous ovarian cancers and that correlated with elevated gene expression of <jats:italic>B4<jats:styled-content style=\"fixed-case\">GALNT<\/jats:styled-content>3<\/jats:italic> and <jats:italic>B4<jats:styled-content style=\"fixed-case\">GALNT<\/jats:styled-content>4<\/jats:italic> in patients with serous cancer. Statistical evaluation of the discriminatory glycans also revealed 13\u00a0<jats:italic>N<\/jats:italic>\u2010 and 3 <jats:italic>O<\/jats:italic>\u2010glycans (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) that significantly discriminated tumour\u2010sampling sites, with Lacdi<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>c\u2010type <jats:italic>N<\/jats:italic>\u2010glycans (<jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 1205.0<jats:sup>2\u2212<\/jats:sup> and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 1059.4<jats:sup>2\u2212<\/jats:sup>) being associated with ovarian\u2010derived cancer tissue and bisecting Glc<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>c\u2010type (<jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 994.9<jats:sup>2\u2212<\/jats:sup>) and branched <jats:italic>N<\/jats:italic>\u2010glycans (<jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 1294.0<jats:sup>2\u2212<\/jats:sup> and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 1148.4<jats:sup>2\u2212<\/jats:sup>) upregulated at the metastatic sites. Hence, we demonstrate for the first time that <jats:styled-content style=\"fixed-case\">OC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> display distinct molecular signatures at both their glycomic and transcriptomic levels. These signatures may have potential utility for the development of accurate diagnosis and personalized treatments.<\/jats:p>","DOI":"10.1002\/1878-0261.12134","type":"journal-article","created":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T17:16:44Z","timestamp":1504027004000},"page":"1595-1615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Tissue glycomics distinguish tumour sites in women with advanced serous adenocarcinoma"],"prefix":"10.1002","volume":"11","author":[{"given":"Merrina","family":"Anugraham","sequence":"first","affiliation":[{"name":"Department of Chemistry &amp; Biomolecular Sciences Biomolecular Discovery &amp; Design Research Centre Faculty of Science and Engineering Macquarie University North Ryde NSW Australia"},{"name":"Glyco\u2010oncology Ovarian Cancer Research Department of Biomedicine University Hospital Basel University of Basel 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