{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T10:43:48Z","timestamp":1784630628074,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,26]],"date-time":"2018-01-26T00:00:00Z","timestamp":1516924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003345","name":"Chinese Academy of Meteorological Sciences","doi-asserted-by":"publisher","award":["2016\u2010I2M\u20103\u2010013"],"award-info":[{"award-number":["2016\u2010I2M\u20103\u2010013"]}],"id":[{"id":"10.13039\/501100003345","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:p>CD30 is a 120\u2010kDa type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. Overexpression of CD30 has been reported in Hodgkin's lymphoma (HL) and anaplastic large\u2010cell lymphoma (ALCL). CD30\u2010targeted treatment with antibody\u2013drug conjugates (ADCs) can lead to promising clinical benefit. Lidamycin (LDM), consisting of an apoprotein LDP and an active enediyne chromophore AE, is a member of the enediyne antibiotic family and one of the most potent antitumor agents. AE and LDP can be dissociated and reconstituted under certain conditions <jats:italic>in\u00a0vitro<\/jats:italic>. LDM is an ideal payload for the preparation of ADCs. In this study, we show the generation, production, and antitumor activity of anti\u2010CD30\u2010LDM, a novel ADC which consists of the intact anti\u2010CD30 antibody and LDM. First, the anti\u2010CD30\u2010LDP fusion protein was constructed and expressed in CHO\/dhFr<jats:sup>\u2212<\/jats:sup> cells. Anti\u2010CD30\u2010LDP showed specific and high\u2010affinity binding to CD30 and could be internalized into target cells. It also exhibited excellent tumor\u2010targeting capability <jats:italic>in\u00a0vivo<\/jats:italic>. Next, anti\u2010CD30\u2010LDM was prepared by assembling the enediyne molecule AE to the fusion protein anti\u2010CD30\u2010LDP. Anti\u2010CD30\u2010LDM was highly cytotoxic to HL and ALCL cell lines, with IC<jats:sub>50<\/jats:sub> values of 5\u201350\u00a0p<jats:sc>m<\/jats:sc>. It can also induce cell apoptosis and G2\/M cell cycle arrest. In the Karpas299 xenograft model, the tumor growth was inhibited by 87.76% in mice treated with anti\u2010CD30\u2010LDM and with no discernible adverse effects. Taken together, anti\u2010CD30\u2010LDM shows attractive tumor\u2010targeting capability and antitumor efficacy both <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic> and could be a promising candidate for the treatment of CD30<jats:sup>+<\/jats:sup> lymphomas.<\/jats:p>","DOI":"10.1002\/1878-0261.12166","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T12:01:56Z","timestamp":1515585716000},"page":"339-355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["A novel enediyne\u2010integrated antibody\u2013drug conjugate shows promising antitumor efficacy against CD30<sup>+<\/sup> lymphomas"],"prefix":"10.1002","volume":"12","author":[{"given":"Rong","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences Peking Union Medical College Beijing 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