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Here, we report on a microfluidic device used to facilitate <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45 depletion to enhance the detection of cytogenetic alterations in plasma cells (PCs). Bone marrow samples from 48 patients with <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content> were each divided into two aliquots. One aliquot was subjected to classic flow cytometry and fluorescent <jats:italic>in\u00a0situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>). The other first went through <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:sup>+<\/jats:sup> cell depletion, further enriched by microfluidic size selection. The enriched samples were then analyzed using flow cytometry and <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> and compared to those analyzed using the classic method only. Unlike the traditional method, the microfluidic device removed the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:sup>+<\/jats:sup> leukocytes and specifically selected PCs from the remaining white blood cells. Therefore, the microfluidic method (<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45\u2010<jats:styled-content style=\"fixed-case\">TAC<\/jats:styled-content>s) significantly increased the percentage of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>38<jats:sup>+<\/jats:sup>\/<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>138<jats:sup>+<\/jats:sup> cells to 37.7\u00a0\u00b1\u00a020.4% (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) from 10.3\u00a0\u00b1 8.5% in bone marrow. After the <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45\u2010<jats:styled-content style=\"fixed-case\">TAC<\/jats:styled-content> enrichment, the detection rate of IgH rearrangement, del(13q14), del(17p), and 1q21 gains, rose to 56.3% (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), 37.5% (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), 22.9% (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), and 41.7% (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001), respectively; all rates of detection were significantly increased compared to the classically analyzed samples. In this clinical trial, this microfluidic\u2010assisted assay provided a precise detection of cytogenetic alterations in PCs and improved clinical outcomes.<\/jats:p>","DOI":"10.1002\/1878-0261.12201","type":"journal-article","created":{"date-parts":[[2018,4,11]],"date-time":"2018-04-11T11:40:22Z","timestamp":1523446822000},"page":"1004-1011","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Microfluidic enrichment of plasma cells improves treatment of multiple myeloma"],"prefix":"10.1002","volume":"12","author":[{"given":"Yunjing","family":"Zeng","sequence":"first","affiliation":[{"name":"Division of Periodontology, Diagnostic Sciences &amp; Dental Hygiene Division of Biomedical Sciences Herman Ostrow School of Dentistry University of Southern California Los Angeles CA USA"},{"name":"Department of Hematology Xinqiao Hospital Army Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Gao","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Diagnostic Sciences &amp; Dental Hygiene Division of Biomedical Sciences Herman Ostrow School of Dentistry University of Southern California Los Angeles CA USA"},{"name":"Department of Hematology Xinqiao Hospital Army Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Hematology Xinqiao Hospital Army Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa H.","family":"Kabeer","sequence":"additional","affiliation":[{"name":"Children's Hospital of Orange County University of California\u2010Irvine School of Medicine Orange CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelian","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Diagnostic Sciences &amp; Dental Hygiene Division of Biomedical Sciences Herman Ostrow School of Dentistry University of Southern California Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"Stucky","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Diagnostic Sciences &amp; Dental Hygiene Division of Biomedical Sciences Herman Ostrow School of Dentistry University of Southern California Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William G.","family":"Loudon","sequence":"additional","affiliation":[{"name":"Children's Hospital of Orange County University of California\u2010Irvine School of Medicine Orange CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengwen C.","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Diagnostic Sciences &amp; Dental Hygiene Division of Biomedical Sciences Herman Ostrow School of Dentistry University of Southern California Los Angeles CA USA"},{"name":"Children's Hospital of Orange County University of California\u2010Irvine School of Medicine Orange CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Diagnostic Sciences &amp; Dental Hygiene Division of Biomedical Sciences Herman Ostrow School of Dentistry University of Southern California Los Angeles CA USA"},{"name":"Department of Hematology Xinqiao Hospital Army Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang F.","family":"Zhong","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Diagnostic Sciences &amp; Dental Hygiene Division of Biomedical Sciences Herman Ostrow School of Dentistry University of Southern California Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(00)00625-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sdata.2016.79"},{"key":"e_1_2_8_4_1","first-page":"116","article-title":"Molecular cytogenetic aberrations in patients with multiple myeloma studied by interphase fluorescence in\u00a0situ hybridization","volume":"29","author":"Chen L","year":"2007","journal-title":"Exp 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