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M\u03c6 heterogeneity, the mechanisms of their recruitment, and their differentiation into lymphoma\u2010promoting, alternatively activated M2\u2010like phenotypes are still not fully understood. Therefore, further functional studies are required to understand biological mechanisms associated with human tumor\u2010associated M\u03c6 (TAM). Here, we show that the global mRNA expression and protein abundance of human M\u03c6 differentiated in Hodgkin lymphoma (HL)\u2010conditioned medium (CM) differ from those of M\u03c6 educated by conditioned media from diffuse large B\u2010cell lymphoma (DLBCL) cells or, classically, by macrophage colony\u2010stimulating factor (M\u2010CSF). Conditioned media from HL cells support TAM differentiation through upregulation of surface antigens such as CD40, CD163, CD206, and PD\u2010L1. In particular, RNA and cell surface protein expression of <jats:italic>mannose receptor 1<\/jats:italic> (<jats:italic>MRC1<\/jats:italic>)\/CD206 significantly exceed the levels induced by classical M\u2010CSF stimulation in M2\u2010like M\u03c6; this is regulated by interleukin 13 to a large extent. Functionally, high CD206 enhances mannose\u2010dependent endocytosis and uptake of type I collagen. Together with high matrix metalloprotease9 secretion, HL\u2010TAMs appear to be active modulators of the tumor matrix. Preclinical <jats:italic>in\u00a0ovo<\/jats:italic> models show that co\u2010cultures of HL cells with monocytes or M\u03c6 support dissemination of lymphoma cells via lymphatic vessels, while tumor size and vessel destruction are decreased in comparison with lymphoma\u2010only tumors. Immunohistology of human HL tissues reveals a fraction of cases feature large numbers of CD206\u2010positive cells, with high <jats:italic>MRC1<\/jats:italic> expression being characteristic of HL\u2010stage IV. In summary, the lymphoma\u2010TAM interaction contributes to matrix\u2010remodeling and lymphoma cell dissemination.<\/jats:p>","DOI":"10.1002\/1878-0261.12616","type":"journal-article","created":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T11:04:59Z","timestamp":1576062299000},"page":"571-589","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["High CD206 levels in Hodgkin lymphoma\u2010educated macrophages are linked to matrix\u2010remodeling and lymphoma dissemination"],"prefix":"10.1002","volume":"14","author":[{"given":"Annekatrin","family":"Arlt","sequence":"first","affiliation":[{"name":"Clinic of Hematology and Medical Oncology University Medical Centre G\u00f6ttingen Germany"},{"name":"Network BMBF eMed MMML\u2010Demonstrators Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederike","family":"von Bonin","sequence":"additional","affiliation":[{"name":"Clinic of 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