{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T23:48:38Z","timestamp":1794354518422,"version":"4.9.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T00:00:00Z","timestamp":1581033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p>Oncolytic viruses armed with therapeutic transgenes of interest show great potential in cancer immunotherapy. Here, a novel oncolytic adenovirus carrying a signal regulatory protein\u2010\u03b1 (SIRP\u03b1)\u2010IgG1\u00a0Fc fusion gene (termed SG635\u2010SF) was constructed, which could block the CD47 \u2018don't eat me\u2019 signal of cancer cells. A strong promoter sequence (CCAU) was chosen to control the expression of the SF fusion protein, and a 5\/35 chimeric fiber was utilized to enhance the efficiency of infection. As a result, SG635\u2010SF was found to specifically proliferate in hTERT\u2010positive cancer cells and largely increased the abundance of the SF gene. The SF fusion protein was effectively detected, and CD47 was successfully blocked in SK\u2010OV3 and HO8910 ovarian cancer cells expressing high levels of CD47. Although the ability to induce cell cycle arrest and cell death was comparable to that of the control empty SG635 oncolytic adenovirus <jats:italic>in\u00a0vitro<\/jats:italic>, the antitumor effect of SG635\u2010SF was significantly superior to that of SG635 <jats:italic>in\u00a0vivo<\/jats:italic>. Furthermore, CD47 was largely blocked and macrophage infiltration distinctly increased in xenograft tissues of SK\u2010OV3 cells but not in those of CD47\u2010negative HepG2 cells, indicating that the enhanced antitumor effect of SG635\u2010SF was CD47\u2010dependent. Collectively, these findings highlight a potent antitumor effect of SG635\u2010SF in the treatment of CD47\u2010positive cancers.<\/jats:p>","DOI":"10.1002\/1878-0261.12628","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:35:15Z","timestamp":1578065715000},"page":"657-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["A SIRP\u03b1\u2010Fc fusion protein enhances the antitumor effect of oncolytic adenovirus against ovarian cancer"],"prefix":"10.1002","volume":"14","author":[{"given":"Yao","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biliary Tract Shanghai Eastern Hepatobiliary Surgery Hospital China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai\u2010qun","family":"Lv","sequence":"additional","affiliation":[{"name":"Laboratory of Viral and Gene Therapy Shanghai Eastern Hepatobiliary Surgery Hospital 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