{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:07Z","timestamp":1788307207983,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,19]],"date-time":"2020-08-19T00:00:00Z","timestamp":1597795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802077"],"award-info":[{"award-number":["81802077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>Osteosarcoma is an uncommon tumor occurring in bone, accompanied by elevated incidence and reduced rate of healing. Epithelial\u2010to\u2010mesenchymal transition (EMT) serves as a conceptual paradigm to explain the invasion and metastasis of osteosarcoma and other cancers. Hence, developing effective therapeutic strategy to treat the EMT of osteosarcoma is essential. Here, we identified the molecular mechanism of long noncoding RNA (lncRNA) PGM5\u2010AS1 in EMT and progression of osteosarcoma. Microarray\u2010based analysis was employed to screen the osteosarcoma\u2010related differentially expressed lncRNAs. The levels of PGM5\u2010AS1 as well as microRNA\u2010140\u20105p (miR\u2010140\u20105p) and fibrillin\u20101 (FBN1) in osteosarcoma tissues and cells were determined. Dual\u2010luciferase reporter gene assay, RNA pull\u2010down assay, and RNA immunoprecipitation assay were conducted to validate the relationship among PGM5\u2010AS1, miR\u2010140\u20105p, and FBN1. Expression of PGM5\u2010AS1, miR\u2010140\u20105p, and FBN1 was altered by overexpression, shRNA, mimic, or inhibitors in order to investigate how they regulated migration, invasion, and EMT of osteosarcoma cells <jats:italic>in\u00a0vitro<\/jats:italic>. Loss\u2010 and gain\u2010of\u2010function approaches were employed in nude mice to detect their roles in tumorigenesis <jats:italic>in\u00a0vivo<\/jats:italic>. Osteosarcoma tissues and cells exhibited low expression of miR\u2010140\u20105p, but high expression of PGM5\u2010AS1 and FBN1. PGM5\u2010AS1 competitively bound to miR\u2010140\u20105p to upregulate FBN1. Furthermore, hindering PGM5\u2010AS1 and FBN1 or overexpressing miR\u2010140\u20105p dampened migration, invasion, and EMT of osteosarcoma cells <jats:italic>in\u00a0vitro<\/jats:italic>. Furthermore, silencing PGM5\u2010AS1 or FBN1, or overexpressing miR\u2010140\u20105p markedly inhibited tumorigenesis in nude mice <jats:italic>in\u00a0vivo<\/jats:italic>. Taken together, PGM5\u2010AS1 depletion causes FBN1 reduction to retard osteosarcoma processes by negatively modulating miR\u2010140\u20105p.<\/jats:p>","DOI":"10.1002\/1878-0261.12711","type":"journal-article","created":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T05:08:57Z","timestamp":1589605737000},"page":"2660-2677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["<i>Retracted:<\/i> Long non\u2010coding RNA PGM5\u2010AS1 promotes epithelial\u2010mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR\u2010140\u20105p\u2010mediated FBN1 inhibition"],"prefix":"10.1002","volume":"14","author":[{"given":"Wei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Spine Surgery The First Hospital of Jilin University Changchun 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