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To improve upon cell line models of HGSOC, we set out to characterize a panel of patient\u2010derived cells and determine their epithelial and mesenchymal characteristics. We analyzed RNA and protein expression levels in patient\u2010derived xenograft (PDX) models of HGSOC, and functionally characterized these models using flow cytometry, wound healing assays, invasion assays, and spheroid cultures. Besides\n                    <jats:italic>in\u00a0vitro<\/jats:italic>\n                    work, we also evaluated the growth characteristics of PDX\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    (orthotopic PDX). We found that all samples had hybrid characteristics, covering a spectrum from an epithelial\u2010to\u2010mesenchymal state. Samples with a stronger epithelial phenotype were more active in self\u2010renewal assays and more tumorigenic in orthotopic xenograft models as compared to samples with a stronger mesenchymal phenotype, which were more migratory and invasive. Additionally, we observed an inverse association between microRNA\n                    <jats:italic>let\u20107<\/jats:italic>\n                    (\n                    <jats:italic>lethal\u20107<\/jats:italic>\n                    ) expression and stemness, consistent with the loss of\n                    <jats:italic>let\u20107<\/jats:italic>\n                    being an important component of the cancer stem cell phenotype. We observed that lower\n                    <jats:italic>let\u20107<\/jats:italic>\n                    levels were associated with the epithelial state and a lower epithelial mesenchymal transition (EMT) score, more efficient spheroid and tumor formation, and increased sensitivity to platinum\u2010based chemotherapy. Surprisingly, in these HGSOC cells, stemness could be dissociated from invasiveness: Cells with lower\n                    <jats:italic>let\u20107<\/jats:italic>\n                    levels were more tumorigenic, but less migratory, and with a lower EMT score, than those with higher\n                    <jats:italic>let\u20107<\/jats:italic>\n                    levels. We conclude that\n                    <jats:italic>let\u20107<\/jats:italic>\n                    expression and epithelial\/mesenchymal state are valuable predictors of HGSOC proliferation,\n                    <jats:italic>in\u00a0vitro<\/jats:italic>\n                    self\u2010renewal, and tumor burden\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.12762","type":"journal-article","created":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T15:28:53Z","timestamp":1594481333000},"page":"2796-2813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Epithelial\/mesenchymal heterogeneity of high\u2010grade serous ovarian carcinoma samples correlates with miRNA let\u20107 levels and predicts tumor growth and metastasis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3301-2724","authenticated-orcid":false,"given":"Evgeny","family":"Chirshev","sequence":"first","affiliation":[{"name":"Division of Biochemistry Department of Basic Sciences Loma Linda University Loma Linda CA USA"},{"name":"Department of Pathology and Human Anatomy Loma Linda University School of Medicine Loma Linda CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomi","family":"Hojo","sequence":"additional","affiliation":[{"name":"Division of Biochemistry Department of Basic Sciences Loma Linda University Loma Linda CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Bertucci","sequence":"additional","affiliation":[{"name":"Division of Biochemistry Department of Basic Sciences Loma Linda University Loma Linda CA 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