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During their flotation, anchorage\u2010dependent epithelial\u2010type tumor cells gain anoikis resistance, implicating integrins, including \u03b1v\u00df3. In this study, we explored anoikis escape, cisplatin resistance, and prosurvival signaling as a function of the \u03b1v\u00df3 transmembrane conformational activation state in cells suspended in ascites. A high\u2010affinity and constitutively signaling\u2010competent \u03b1v\u00df3 variant, which harbored unclasped transmembrane domains, was found to confer delayed anoikis onset, enhanced cisplatin resistance, and reduced cell proliferation in ascites or 3D\u2010hydrogels, involving p27<jats:sup>kip<\/jats:sup> upregulation. Moreover, it promoted EGF\u2010R expression and activation, prosurvival signaling, implicating FAK, src, and PKB\/Akt. This led to the induction of the anti\u2010apoptotic factors Bcl\u20102 and survivin suppressing caspase activation, compared to a signaling\u2010incapable \u03b1v\u00df3 variant displaying firmly associated transmembrane domains. Dissecting the mechanistic players for \u03b1v\u00df3\u2010dependent survival and peritoneal metastasis of ascitic ovarian cancer spheroids is of paramount importance to target their anchorage independence by reversing anoikis resistance and blocking \u03b1v\u00df3\u2010triggered prosurvival signaling.<\/jats:p>","DOI":"10.1002\/1878-0261.12845","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T08:49:09Z","timestamp":1604652549000},"page":"503-522","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Constitutive activation of integrin \u03b1v\u03b23 contributes to anoikis resistance of ovarian cancer cells"],"prefix":"10.1002","volume":"15","author":[{"given":"Romana","family":"Dolinschek","sequence":"first","affiliation":[{"name":"Department for Obstetrics &amp; Gynecology Clinical Research Unit Technische Universit\u00e4t M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Hingerl","sequence":"additional","affiliation":[{"name":"Department for Obstetrics &amp; Gynecology Clinical Research Unit Technische Universit\u00e4t M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke","family":"Benge","sequence":"additional","affiliation":[{"name":"Department for Obstetrics &amp; Gynecology Clinical Research Unit Technische Universit\u00e4t M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Zafiu","sequence":"additional","affiliation":[{"name":"Department of Water, Atmosphere, and Environment University for Natural Resources and Applied Life Sciences (BOKU) Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Sch\u00fcren","sequence":"additional","affiliation":[{"name":"Department for Obstetrics &amp; Gynecology Clinical Research Unit Technische Universit\u00e4t M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva\u2010Kathrin","family":"Ehmoser","sequence":"additional","affiliation":[{"name":"Department for Nanobiotechnology Institute for Synthetic Bioarchitectures University for Natural Resources and Applied Life Sciences (BOKU) Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"L\u00f6ssner","sequence":"additional","affiliation":[{"name":"Faculties of Engineering and Medicine Nursing &amp; Health Sciences Monash University Melbourne Vic. 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