{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T01:49:29Z","timestamp":1783648169525,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,5,29]],"date-time":"2021-05-29T00:00:00Z","timestamp":1622246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["836041013"],"award-info":[{"award-number":["836041013"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:p>\n                    Circulating RNAs extracted from liquid biopsies represent a promising source of cancer\u2010 and therapy\u2010related biomarkers. We screened whole blood from patients with metastatic castration\u2010resistant prostate cancer (mCRPC) following their first\u2010line treatment with abiraterone acetate and prednisone (AA\u2010P) to identify circulating RNAs that may correlate with progression\u2010free survival (PFS). In a prospective multicenter observational study, 53 patients with mCRPC were included after they started first\u2010line AA\u2010P treatment. Blood was drawn at baseline, 1, 3, and 6\u00a0months after treatment initiation. The levels of predefined circulating RNAs earlier identified as being upregulated in patients with mCRPC (e.g., microRNAs, long noncoding RNAs, and mRNAs), were analyzed. Uni\u2010 and multivariable Cox regression and Kaplan\u2013Meier analyses were used to analyze the prognostic value of the various circulating RNAs for PFS along treatment. Detectable levels of kallikrein\u2010related peptidase 3 (\n                    <jats:italic>KLK3<\/jats:italic>\n                    ) mRNA at baseline were demonstrated to be an independent prognostic marker for PFS (201 vs 501\u00a0days,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.00054). Three months after AA\u2010P treatment initiation,\n                    <jats:italic>KLK3<\/jats:italic>\n                    could not be detected in the blood of responding patients, but was still detectable in 56% of the patients with early progression. Our study confirmed that\n                    <jats:italic>KLK3<\/jats:italic>\n                    mRNA detection in whole blood is an independent prognostic marker in mCRPC patients receiving AA\u2010P treatment. Furthermore, the levels of circulating\n                    <jats:italic>KLK3<\/jats:italic>\n                    mRNA in patients receiving AA\u2010P treatment might reflect treatment response or early signs of progression.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.12933","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T04:09:25Z","timestamp":1614658165000},"page":"2453-2465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Liquid biopsy reveals KLK3 mRNA as a prognostic marker for progression free survival in patients with metastatic castration\u2010resistant prostate cancer undergoing first\u2010line abiraterone acetate and prednisone treatment"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3041-6774","authenticated-orcid":false,"given":"Emmy","family":"Boerrigter","sequence":"first","affiliation":[{"name":"Department of Pharmacy Radboud University Medical Center Radboud Institute for Health Sciences Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillemette E.","family":"Benoist","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Radboud University Medical Center Radboud Institute for Health Sciences Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge M.","family":"van Oort","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Medical Center Radboud Institute for Molecular Life Sciences Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald W.","family":"Verhaegh","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Medical Center Radboud Institute for Molecular Life Sciences Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1258-2146","authenticated-orcid":false,"given":"Onno","family":"van Hooij","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Medical Center Radboud Institute for Molecular Life Sciences Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levi","family":"Groen","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Medical Center Radboud Institute for Molecular Life Sciences Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Smit","sequence":"additional","affiliation":[{"name":"MDxHealth Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irma M.","family":"Oving","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Ziekenhuisgroep Twente Almelo the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"de Mol","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Gelderse Vallei Hospital Ede the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tineke J.","family":"Smilde","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Jeroen Bosch Hospital \u2018s Hertogenbosch the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5277-6586","authenticated-orcid":false,"given":"Diederik M.","family":"Somford","sequence":"additional","affiliation":[{"name":"Department of Urology Canisius Wilhelmina Hospital Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niven","family":"Mehra","sequence":"additional","affiliation":[{"name":"Deparment of Medical Oncology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack A.","family":"Schalken","sequence":"additional","affiliation":[{"name":"Department of Urology Radboud University Medical Center Radboud Institute for Molecular Life Sciences Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nielka P.","family":"van Erp","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Radboud University Medical Center Radboud Institute for Health Sciences Nijmegen the 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