{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T14:53:46Z","timestamp":1791816826832,"version":"4.3.4"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T00:00:00Z","timestamp":1616544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:p>\n                    In many solid tumors including triple\u2010negative breast cancer (TNBC), upregulation of the interleukin\u20104 receptor (IL\u20104R) has been shown to promote cancer cell proliferation, apoptotic resistance, metastatic potential, and a Th2 response in the tumor microenvironment (TME). Since immunosuppressive cells in the TME and spleen including myeloid\u2010derived suppressor cells (MDSCs) and tumor\u2010associated macrophages (TAMs) also express the IL\u20104R, we hypothesized that selective depletion of IL\u20104R\u2010bearing cells in TNBC would result in the direct killing of tumor cells and the depletion of immunosuppressive cells and lead to an enhanced antitumor response. To selectively target IL\u20104R\n                    <jats:sup>+<\/jats:sup>\n                    cells, we employed DABIL\u20104, a fusion protein toxin consisting of the catalytic and translocation domains of diphtheria toxin fused to murine IL\u20104. As anticipated, DABIL\u20104 has potent cytotoxic activity against TNBC cells both\n                    <jats:italic>in\u00a0vitro<\/jats:italic>\n                    and\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    . We demonstrate in the murine 4T1 TNBC model that DABIL\u20104 significantly reduces tumor growth, splenomegaly, and lung metastases. Importantly, we also show that the administration of DABIL\u20104 results in the selective depletion of MDSCs, TAMs, and regulatory T cells in treated mice, with a concomitant increase in IFN\u2010\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    CD8 effector T cells in the TME. Since the 4T1 antitumor activity of DABIL\u20104 was largely diminished in IL\u20104R knockout mice, we postulate that DABIL\u20104 functions primarily as an immunotherapeutic by the depletion of MDSCs, TAMs, and regulatory T cells. NanoString analysis of control and treated tumors confirmed and extended these observations by showing a marked decline of mRNA transcripts that are associated with tumorigenesis and metastasis. In conclusion, we demonstrate that DABIL\u20104 targeting of both tumor and immunosuppressive host cells likely represents a novel and effective treatment strategy for 4T1 TNBC and warrants further study.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.12938","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T02:51:53Z","timestamp":1615171913000},"page":"1330-1344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Therapeutic targeting with DABIL\u20104 depletes myeloid suppressor cells in 4T1 triple\u2010negative breast cancer model"],"prefix":"10.1002","volume":"15","author":[{"given":"Sadiya","family":"Parveen","sequence":"first","affiliation":[{"name":"Department of Medicine Division of Infectious Diseases Johns Hopkins University School of Medicine Baltimore MD 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