{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:22:29Z","timestamp":1791390149416,"version":"4.3.3"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p>\n                    Ovarian cancer disseminates primarily intraperitoneally. Detached tumor cell aggregates (spheroids) from the primary tumor are regarded as \u2018metastatic units\u2019 that exhibit a low sensitivity to classical chemotherapy, probably due to their unique molecular characteristics. We have analyzed the cellular composition of ascites from OvCa patients, using flow cytometry, and studied their behavior\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    and\n                    <jats:italic>in\u2009vivo<\/jats:italic>\n                    . We conclude that ascites\u2010derived cultured cells from OvCa patients give rise to two subpopulations: adherent cells and non\u2010adherent cells. Here, we found that the AD population includes mainly CD90\n                    <jats:sup>+<\/jats:sup>\n                    cells with highly proliferative rates\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    but no tumorigenic potential\n                    <jats:italic>in\u2009vivo<\/jats:italic>\n                    , whereas the NAD population contains principally tumor cell spheroids (EpCAM\n                    <jats:sup>+<\/jats:sup>\n                    \/CD24\n                    <jats:sup>+<\/jats:sup>\n                    ) with low proliferative potential\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    . Enriched tumor cell spheroids from the ascites of high\u2010grade serous OvCA patients, obtained using cell strainers, were highly tumorigenic\n                    <jats:italic>in\u2009vivo<\/jats:italic>\n                    and their metastatic spread pattern precisely resembled the tumor dissemination pattern found in the corresponding patients. Comparative transcriptome analyses from ascites\u2010derived tumor cell spheroids (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200910) versus tumor samples from different metastatic sites (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200930) revealed upregulation of genes involved in chemoresistance (\n                    <jats:italic>TGM1<\/jats:italic>\n                    , HSPAs, MT1s), cell adhesion and cell\u2010barrier integrity (\n                    <jats:italic>PKP3<\/jats:italic>\n                    , CLDNs,\n                    <jats:italic>PPL<\/jats:italic>\n                    ), and the oxidative phosphorylation process. Mitochondrial markers (mass and membrane potential) showed a reduced mitochondrial function in tumoroids from tumor tissue compared with ascites\u2010derived tumor spheroids in flow cytometry analysis. Interestingly, response to OXPHOS inhibition by metformin and IACS010759 in tumor spheroids correlated with the extent of mitochondrial membrane potential measured by fluorescence\u2010activated cell sorting. Our data contribute to a better understanding of the biology of ovarian cancer spheroids and identify the OXPHOS pathway as new potential treatment option in advanced ovarian cancer.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13028","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T05:46:11Z","timestamp":1622526371000},"page":"3578-3595","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Molecular characteristics and tumorigenicity of ascites\u2010derived tumor cells: mitochondrial oxidative phosphorylation as a novel therapy target in ovarian cancer"],"prefix":"10.1002","volume":"15","author":[{"given":"Yi","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Gynecology University Medical Center Hamburg\u2010Eppendorf 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