{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:28:48Z","timestamp":1790692128467,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p>The exact role of malignant ascites in the development of intraperitoneal metastases remains unclear, and the mechanisms by which extracellular vesicles (EVs) promote tumor progression in the pre\u2010metastatic niche have not been fully discovered. In this study, we characterized ascites from high\u2010grade epithelial ovarian cancer patients. Small\u2010EVs (30\u2013150\u2009nm) were isolated from two sources\u2014the bulk ascites and the ascitic fluid\u2010derived tumor cell cultures\u2014and assessed with a combination of imaging, proteomic profiling, and protein expression analyses. In addition, Gene Ontology and pathway analysis were performed using different databases and bioinformatic tools. The results proved that the small\u2010EVs derived from the two sources exhibited significantly different stiffness and size distributions. The bulk ascitic fluid\u2010derived small\u2010EVs were predominantly involved in the complement and coagulation cascade. Small\u2010EVs derived from ascites cell cultures contained a robust proteomic profile of extracellular matrix remodeling regulators, and we observed an increase in transforming growth factor\u2010\u03b2\u2010I (TGF\u03b2I), plasminogen activator inhibitor 1 (PAI\u20101), and fibronectin expression after neoadjuvant chemotherapy. When measured in the two sources, we demonstrated that fibronectin exhibited opposite expression patterns in small\u2010EVs in response to chemotherapy. These findings highlight the importance of an ascites cell isolation workflow in investigating the treatment\u2010induced cancer adaption processes.<\/jats:p>","DOI":"10.1002\/1878-0261.13110","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T11:32:10Z","timestamp":1633692730000},"page":"3596-3614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Small extracellular vesicles from malignant ascites of patients with advanced ovarian cancer provide insights into the dynamics of the extracellular matrix"],"prefix":"10.1002","volume":"15","author":[{"given":"Barbara","family":"Bortot","sequence":"first","affiliation":[{"name":"Department of Medical Genetics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maura","family":"Apollonio","sequence":"additional","affiliation":[{"name":"Pediatric Department Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Rampazzo","sequence":"additional","affiliation":[{"name":"Department of Chemistry \"Giacomo Ciamician\" University of Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Valle","sequence":"additional","affiliation":[{"name":"Consorzio Sistemi a Grande Interfase Department of Chemistry University of Firenze Italy"},{"name":"Consiglio Nazionale delle Ricerche Istituto per lo Studio dei Materiali Nanostrutturati (CNRISMN) Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Brucale","sequence":"additional","affiliation":[{"name":"Consorzio Sistemi a Grande Interfase Department of Chemistry University of Firenze Italy"},{"name":"Consiglio Nazionale delle Ricerche Istituto per lo Studio dei Materiali Nanostrutturati (CNRISMN) Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ridolfi","sequence":"additional","affiliation":[{"name":"Consorzio Sistemi a Grande Interfase Department of Chemistry University of Firenze Italy"},{"name":"Department of Chemistry University of Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blendi","family":"Ura","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Addobbati","sequence":"additional","affiliation":[{"name":"Department of Clinical Toxicology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Di Lorenzo","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Romano","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Buonomo","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Ripepi","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Ricci","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"},{"name":"Clinical Department of Medical Surgical and Health Sciences University of Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2257-2053","authenticated-orcid":false,"given":"Stefania","family":"Biffi","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.21421"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.26345"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62146-7"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1123"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2018.29.e66"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13116-2"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.100105"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.03.002"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-1049-4"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.31774"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-0991-5"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2012.02.022"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.02.037"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1596"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2595-9"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2013_071"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1996.0106"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3432"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046858"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2013.07.016"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12051175"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.328"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1555419"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b05716"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.468"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21239291"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/ijgc-2020-001718"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-160614"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.30699\/ijp.13.1.23"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jmi.12910"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3743-1_2"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.16901"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74778"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00641"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2492"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12855"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13403"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002752200"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.10.027"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2013.00256"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-018-0391-2"},{"key":"e_1_2_11_44_1","first-page":"749","article-title":"Exosomes in the ascites of ovarian cancer patients: origin and effects on anti\u2010tumor immunity","volume":"25","author":"Peng P","year":"2011","journal-title":"Oncol Rep"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-88163-1"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12749"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.60.5212"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-020-0875-x"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00605"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2019.129486"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11061476"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-021-00551-z"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/nano.202000129"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b01181"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.29685"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18794-x"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2017.6"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15756"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2019.02.026"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2014.04.004"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.13110","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1878-0261.13110","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.13110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T21:23:41Z","timestamp":1724361821000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1878-0261.13110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,27]]},"references-count":59,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/1878-0261.13110"],"URL":"https:\/\/doi.org\/10.1002\/1878-0261.13110","archive":["Portico"],"relation":{},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,27]]},"assertion":[{"value":"2021-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}