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Viability assay and flow cytometric assay for determining apoptosis, cell cycle, mitochondrial membrane potential, reactive oxygen species and immunoblotting were used to explore the combination effect in DLBCL cell lines, DLBCL patient samples, and DLBCL mouse models. RNA sequencing assay helped identify mechanisms of ABT\u2010199 plus Apatinib. The results show that ABT\u2010199 combined with Apatinib inhibited cell proliferation, reduced colony\u2010forming capacity, and induced apoptosis and cell cycle arrest in DLBCL cells. Mechanistically, the combination therapy inhibited tumour cell growth and promoted tumour cell death by regulating EDN1 and MAPK\u2010related pathways and activating the intrinsic apoptotic pathway. The effect of the combination therapy was also validated in primary DLBCL blasts and xenograft mouse models. Our findings indicate that Apatinib enhances the chemosensitivity of ABT\u2010199 and might serve as a promising therapeutic strategy for DLBCL.<\/jats:p>","DOI":"10.1002\/1878-0261.13309","type":"journal-article","created":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T15:59:47Z","timestamp":1661702387000},"page":"3735-3753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Apatinib enhances chemosensitivity of <scp>ABT<\/scp>\u2010199 in diffuse large B\u2010cell lymphoma"],"prefix":"10.1002","volume":"16","author":[{"given":"Yuanfei","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Hematology, the First Affiliated Hospital, College of Medicine Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Ye","sequence":"additional","affiliation":[{"name":"Sports Medicine Department, Beijing Key Laboratory of Sports Injuries Peking 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