{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:02:50Z","timestamp":1790622170006,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972536"],"award-info":[{"award-number":["81972536"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>\n                    Tertiary lymphoid structures (TLSs) are formed in long\u2010term chronic inflammation, promoting the local recruitment of lymphocytes, antigen presentation and regulation of immune response, correlated with a better prognosis for cancer patients. Although studies have been conducted to explore methods that accelerate the establishment of TLSs, related research in head and neck squamous cell carcinoma (HNSCC) is still lacking. In this study, we analysed data from The Cancer Genome Atlas and performed immunohistochemical staining analyses of 188 patient samples. The results showed that TLSs promoted the infiltration of immune cells. Patients with TLSs with high infiltration of CD8\n                    <jats:sup>+<\/jats:sup>\n                    cells showed the best prognosis. Since lymphotoxin \u03b1 (LT\u03b1) was significantly increased in tissues with TLSs, we overexpressed LT\u03b1 in SCC7 cells (a mouse\u2010derived HNSCC cell line) and established tongue\u2010tumour\u2010bearing models. The polychromatic observation of tissue sections showed that T\u2010cell aggregation increased in the LT\u03b1 cell group, and a grade 1 TLS was formed on the 12th day after inoculating the cells. Moreover, the tumour volume in the LT\u03b1 group was significantly less than that of the control group, whereas both the number and the proportion of infiltrated CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells were increased. The peripheral CD8\n                    <jats:sup>+<\/jats:sup>\n                    cells in mice were removed, and no difference was observed in tumour size or TLS formation. Remarkably, we found that TLS led to an increase in the antitumour effect by recruiting CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in HNSCC, showing a CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell\u2010dependent antitumour effect. Moreover, LT\u03b1 overexpression in the tumour promoted the formation of TLSs.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13403","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T03:13:26Z","timestamp":1677208406000},"page":"1514-1530","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Tertiary lymphoid structures in head and neck squamous cell carcinoma improve prognosis by recruiting\n                    <scp>CD8<\/scp>\n                    <sup>+<\/sup>\n                    T cells"],"prefix":"10.1002","volume":"17","author":[{"given":"Mengyao","family":"Wang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Basic Science of Stomatology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rundong","family":"Zhai","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Basic Science of Stomatology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Basic Science of Stomatology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Basic Science of Stomatology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Basic Science of Stomatology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Yu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Periodontology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Basic Science of Stomatology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhai","family":"Ye","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Depatment of Oral and Maxillofacial Surgery The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6509-922X","authenticated-orcid":false,"given":"Laikui","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  China"},{"name":"Department of Basic Science of Stomatology The Affiliated Stomatological Hospital of Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032641"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-021-00131-7"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.705614"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01181"},{"issue":"1","key":"e_1_2_11_7_1","article-title":"Tertiary lymphoid structures and B lymphocytes in cancer prognosis and response to immunotherapies","volume":"10","author":"N J","year":"2021","journal-title":"Onco Targets Ther"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-20-0949"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100354"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-362-6_5"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2020-002273"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.09.003"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201309-1611OC"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2021.09.021"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.648812"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-020-00092-3"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23355-x"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3836"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00094-2"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-018-0688-7"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80588-9"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1914-8"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058-017-0864-z"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.03.007"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-7"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0114-2"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2021.105964"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13194912"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.61.1509"},{"issue":"10","key":"e_1_2_11_30_1","article-title":"Treatment\u2010na\u00efve HPV+ head and neck cancers display a T\u2010cell\u2010inflamed phenotype distinct from their HPV\u2010 counterparts that has implications for immunotherapy","volume":"7","author":"Gameiro S","year":"2018","journal-title":"Onco Targets Ther"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13194836"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1922-8"},{"issue":"1","key":"e_1_2_11_33_1","article-title":"Tertiary lymphoid structure signatures are associated with survival and immunotherapy response in muscle\u2010invasive bladder cancer","volume":"10","author":"Zhou L","year":"2021","journal-title":"Onco Targets Ther"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.673042"},{"issue":"1","key":"e_1_2_11_35_1","article-title":"Single\u2010cell profiling of infiltrating B cells and tertiary lymphoid structures in the TME of gastric adenocarcinomas","volume":"10","author":"Jia L","year":"2021","journal-title":"Onco Targets Ther"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-021-09792-8"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-19-2929"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13020284"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-019-0235-4"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.04.026"},{"issue":"1","key":"e_1_2_11_41_1","first-page":"171","article-title":"Immunological potential of tertiary lymphoid structures surrounding the primary tumour in gastric cancer","volume":"57","author":"Yamakoshi Y","year":"2020","journal-title":"Int J Oncol"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500686"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13081775"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.30845"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2882-8"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3376"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24633"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-0952"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.13403","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1878-0261.13403","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.13403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:39:01Z","timestamp":1692319141000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1878-0261.13403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,8]]},"references-count":47,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/1878-0261.13403"],"URL":"https:\/\/doi.org\/10.1002\/1878-0261.13403","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v3\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13403\/v1\/review1","asserted-by":"object"}]},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,8]]},"assertion":[{"value":"2022-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}