{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T23:59:18Z","timestamp":1784937558005,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:00:00Z","timestamp":1679529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["765492"],"award-info":[{"award-number":["765492"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:p>Alternative sources of tumour information need to be explored in patients with non\u2010small cell lung cancer (NSCLC). Here, we compared programmed cell death ligand 1 (<jats:italic>PD\u2010L1<\/jats:italic>) expression on cytology imprints and circulating tumour cells (CTCs) with PD\u2010L1 tumour proportion score (TPS) from immunohistochemistry staining of tumour tissue from patients with NSCLC. We evaluated <jats:italic>PD\u2010L1<\/jats:italic> expression using a PD\u2010L1 antibody (28\u20108) in representative cytology imprints, and tissue samples from the same tumour. We report good agreement rates on PD\u2010L1 positivity (TPS\u2009\u2265\u20091%) and high <jats:italic>PD\u2010L1<\/jats:italic> expression (TPS\u2009\u2265\u200950%). Considering high <jats:italic>PD\u2010L1<\/jats:italic> expression, cytology imprints showed a PPV of 64% and a NPV of 85%. CTCs were detected in 40% of the patients and 80% of them were PD\u2010L1<jats:sup>+<\/jats:sup>. Seven patients with <jats:italic>PD\u2010L1<\/jats:italic> expression of &lt;\u20091% in tissue samples or cytology imprints had PD\u2010L1<jats:sup>+<\/jats:sup> CTCs. The addition of <jats:italic>PD\u2010L1<\/jats:italic> expression in CTCs to cytology imprints markedly improved the prediction capacity for PD\u2010L1 positivity. A combined analysis of cytological imprints and CTCs provides information on the tumoural PD\u2010L1 status in NSCLC patients, which might be used when no tumor tissue is available.<\/jats:p>","DOI":"10.1002\/1878-0261.13415","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T13:13:35Z","timestamp":1678367615000},"page":"737-746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Comparative evaluation of <scp><i>PD\u2010L1<\/i><\/scp> expression in cytology imprints, circulating tumour cells and tumour tissue in non\u2010small cell lung cancer patients"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3300-1398","authenticated-orcid":false,"given":"Mustafa","family":"Abdo","sequence":"first","affiliation":[{"name":"LungenClinic Grosshansdorf, Airway Research Center North German Center for Lung Research  Grosshansdorf 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