{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:15:01Z","timestamp":1792721701750,"version":"4.5.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T00:00:00Z","timestamp":1682294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:p>\n                    The utility of multigene expression assays in advanced (\u2265\u20094 positive lymph nodes) early breast cancer (EBC) is limited. We conducted exploratory transcriptomic analysis of 758 genes (Breast Cancer 360 panel, nCounter\n                    <jats:sup>\u00ae<\/jats:sup>\n                    platform; NanoString) in primary tumor samples collected during a phase 3 trial comparing adjuvant taxane\u2010containing dose\u2010dense chemotherapy (ddCTX) versus standard\u2010dosed chemotherapy (stCTX) in resected EBC with \u2265\u20094 positive lymph nodes. Prognostic and predictive associations with disease\u2010free survival (DFS) and overall survival (OS) were evaluated by Cox regression with false discovery rate (FDR) adjustment. Data were available from tumor samples of 141\/226 patients (median follow\u2010up: 14\u2009years). Several genes\/signatures, including immune markers, showed prognostic relevance in unadjusted analyses. Of these, two remained significant after multiplicity adjustment: a positive effect on DFS of programmed cell death 1 ligand\u20102 (PD\u2010L2) in the ddCTX arm (univariate HR: 0.53, FDR\u2010adjusted\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.036) and a negative effect on OS of HER2\u2010enriched (HER2\u2010E) signature in the stCTX arm (univariate HR: 5.40, FDR\u2010adjusted\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.036). Predictive analyses showed greater DFS benefit of ddCTX in tumors with high antigen processing machinery (APM) expression (multivariate interaction\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.024). Multigene expression assays have a prognostic and predictive potential in advanced EBC, and further investigation is warranted in order to identify candidates for de\u2010escalated treatment. In addition, intrinsic subtype and immune gene expression have predictive potential.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13435","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T06:24:46Z","timestamp":1681453486000},"page":"1060-1075","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prognostic and predictive impact of gene expression in node\u2010positive early breast cancer patients receiving dose\u2010dense versus standard\u2010dose adjuvant chemotherapy"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1381-8595","authenticated-orcid":false,"given":"Mattea","family":"Reinisch","sequence":"first","affiliation":[{"name":"Breast Unit Kliniken Essen\u2010Mitte  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Bruzas","sequence":"additional","affiliation":[{"name":"Breast Unit Kliniken Essen\u2010Mitte  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg","family":"Gluz","sequence":"additional","affiliation":[{"name":"Bethesda Hospital Breast Center Niederrhein  M\u00f6nchengladbach Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beyhan","family":"Ataseven","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Gynecologic Oncology Kliniken Essen\u2010Mitte  Germany"},{"name":"Department of Obstetrics and Gynecology University Hospital, LMU  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Schmid","sequence":"additional","affiliation":[{"name":"Centre for Experimental Cancer Medicine, Barts Cancer Institute Queen Mary University of London  UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Cort\u00e9s","sequence":"additional","affiliation":[{"name":"International Breast Cancer Centre (IBCC) Quiron Group  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Essen\u2010Mitte  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ouafaa","family":"Chiari","sequence":"additional","affiliation":[{"name":"Breast Unit Kliniken Essen\u2010Mitte  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakima","family":"Harrach","sequence":"additional","affiliation":[{"name":"Breast Unit Kliniken Essen\u2010Mitte  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Gebauer","sequence":"additional","affiliation":[{"name":"Institute for Pathology Viersen  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Traut","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Gynecologic Oncology Kliniken Essen\u2010Mitte  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherko","family":"Kuemmel","sequence":"additional","affiliation":[{"name":"Breast Unit Kliniken Essen\u2010Mitte  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